Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 103709..104436 | Replicon | plasmid pVir_020079 |
| Accession | NZ_CP029383 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain SCKP020079 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | CLQ72_RS02430 | Protein ID | WP_011251285.1 |
| Coordinates | 103709..104020 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | CLQ72_RS02435 | Protein ID | WP_011251286.1 |
| Coordinates | 104017..104436 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CLQ72_RS02385 | 98720..99230 | + | 511 | Protein_119 | hypothetical protein | - |
| CLQ72_RS02390 | 99220..99660 | + | 441 | WP_011251275.1 | hypothetical protein | - |
| CLQ72_RS02395 | 99690..99881 | + | 192 | Protein_121 | hypothetical protein | - |
| CLQ72_RS02400 | 100085..101041 | - | 957 | WP_011251280.1 | DsbA family protein | - |
| CLQ72_RS02405 | 101101..101442 | - | 342 | WP_011251281.1 | hypothetical protein | - |
| CLQ72_RS02410 | 101456..101767 | - | 312 | WP_011251282.1 | hypothetical protein | - |
| CLQ72_RS02415 | 101784..102218 | - | 435 | Protein_125 | thiol:disulfide interchange protein | - |
| CLQ72_RS02420 | 102235..102932 | - | 698 | Protein_126 | IS1 family transposase | - |
| CLQ72_RS02425 | 103046..103504 | + | 459 | Protein_127 | IS3 family transposase | - |
| CLQ72_RS02430 | 103709..104020 | + | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| CLQ72_RS02435 | 104017..104436 | + | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| CLQ72_RS02440 | 104382..104624 | - | 243 | WP_108970991.1 | hypothetical protein | - |
| CLQ72_RS02445 | 104628..105551 | + | 924 | WP_048333456.1 | IS5 family transposase | - |
| CLQ72_RS02450 | 105653..105988 | - | 336 | Protein_132 | conjugal transfer protein TraI | - |
| CLQ72_RS02455 | 106002..106790 | - | 789 | WP_040217257.1 | hypothetical protein | - |
| CLQ72_RS02460 | 106811..107254 | - | 444 | WP_048333569.1 | hypothetical protein | - |
| CLQ72_RS02465 | 107850..108120 | + | 271 | Protein_135 | hypothetical protein | - |
| CLQ72_RS02470 | 108192..108485 | + | 294 | WP_049126232.1 | hypothetical protein | - |
| CLQ72_RS02475 | 108798..109268 | + | 471 | WP_048333570.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN | 1..178871 | 178871 | |
| - | inside | IScluster/Tn | - | - | 96800..121107 | 24307 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T105555 WP_011251285.1 NZ_CP029383:103709-104020 [Klebsiella pneumoniae subsp. pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T105555 NZ_CP029383:103709-104020 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT105555 WP_011251286.1 NZ_CP029383:104017-104436 [Klebsiella pneumoniae subsp. pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT105555 NZ_CP029383:104017-104436 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|