Detailed information of TA system TA105549

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 145694..145947
• Replicon: plasmid pKPC2_020079
• Accession: NZ_CP029381
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain SCKP020079

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: CLQ72_RS01270
• Protein ID: WP_001312851.1
• Coordinates: 145798..145947 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	145694..145753 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ72_RS01215	141017..141130	+	114	Protein_216	adenine-specific DNA-methyltransferase	-
CLQ72_RS01220	141076..141462	-	387	WP_109491564.1	DDE-type integrase/transposase/recombinase	-
CLQ72_RS01225	141407..141526	+	120	Protein_218	AraC family transcriptional regulator	-
CLQ72_RS01230	141472..142176	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
CLQ72_RS01235	142240..142401	+	162	Protein_220	DNA helicase	-
CLQ72_RS01240	142421..143167	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
CLQ72_RS01245	143222..143782	+	561	WP_000139328.1	fertility inhibition protein FinO	-
CLQ72_RS01250	143914..144114	+	201	WP_015059022.1	hypothetical protein	-
CLQ72_RS01255	144500..145099	+	600	WP_032083981.1	hypothetical protein	-
CLQ72_RS01260	145263..145493	+	231	WP_001736714.1	hypothetical protein	-
CLQ72_RS01265	145516..145743	-	228	Protein_226	hypothetical protein	-
-	145694..145753	-	60	NuclAT_1	-	Antitoxin
-	145694..145753	-	60	NuclAT_1	-	Antitoxin
-	145694..145753	-	60	NuclAT_1	-	Antitoxin
-	145694..145753	-	60	NuclAT_1	-	Antitoxin
CLQ72_RS01270	145798..145947	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
CLQ72_RS01275	146231..146479	+	249	WP_000083837.1	replication regulatory protein RepA	-
CLQ72_RS01280	146480..146689	-	210	WP_064765370.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaKPC-2 / blaSHV-12	-	1..146790	146790

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T105549 WP_001312851.1 NZ_CP029381:145798-145947 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T105549 NZ_CP029381:145798-145947 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT105549 NZ_CP029381:c145753-145694 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin