Detailed information of TA system TA105539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1852288..1852982
• Replicon: chromosome
• Accession: NZ_CP029371
• Organism: Escherichia coli strain C

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: DIZ83_RS09860
• Protein ID: WP_001263493.1
• Coordinates: 1852288..1852686 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: DIZ83_RS09865
• Protein ID: WP_000554757.1
• Coordinates: 1852689..1852982 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DIZ83_RS09830	1847288..1848532	-	1245	WP_000189541.1	esterase FrsA	-
-	1847948..1848028	-	81	NuclAT_13	-	-
-	1847948..1848028	-	81	NuclAT_13	-	-
-	1847948..1848028	-	81	NuclAT_13	-	-
-	1847948..1848028	-	81	NuclAT_13	-	-
DIZ83_RS09835	1848624..1849082	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
DIZ83_RS09840	1849343..1850800	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
DIZ83_RS09845	1850857..1851378	-	522	Protein_1792	peptide chain release factor H	-
DIZ83_RS09850	1851377..1851580	-	204	Protein_1793	RNA ligase RtcB family protein	-
DIZ83_RS09855	1851826..1852278	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
DIZ83_RS09860	1852288..1852686	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
DIZ83_RS09865	1852689..1852982	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
DIZ83_RS09870	1853034..1854089	-	1056	WP_001226164.1	DNA polymerase IV	-
DIZ83_RS09875	1854160..1854945	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
DIZ83_RS09880	1854917..1856629	+	1713	Protein_1799	flagellar biosynthesis protein FlhA	-
DIZ83_RS09885	1856845..1857342	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T105539 WP_001263493.1 NZ_CP029371:c1852686-1852288 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T105539 NZ_CP029371:c1852686-1852288 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT105539 WP_000554757.1 NZ_CP029371:c1852982-1852689 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT105539 NZ_CP029371:c1852982-1852689 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6