Detailed information of TA system TA105511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1110102..1110829
• Replicon: chromosome
• Accession: NZ_CP029369
• Organism: Escherichia coli strain WCHEC035148

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: DI496_RS07425
• Protein ID: WP_000547564.1
• Coordinates: 1110102..1110413 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DI496_RS07430
• Protein ID: WP_000126294.1
• Coordinates: 1110410..1110829 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DI496_RS07395	1105244..1106953	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
DI496_RS07400	1106963..1107505	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
DI496_RS07405	1107505..1108272	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
DI496_RS07410	1108269..1108679	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
DI496_RS07415	1108672..1109142	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
DI496_RS07420	1109167..1109928	+	762	WP_001026446.1	hypothetical protein	-
DI496_RS07425	1110102..1110413	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
DI496_RS07430	1110410..1110829	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
DI496_RS07435	1110943..1112367	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
DI496_RS07440	1112376..1113833	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
DI496_RS07445	1114093..1115103	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
DI496_RS07450	1115252..1115779	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T105511 WP_000547564.1 NZ_CP029369:1110102-1110413 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T105511 NZ_CP029369:1110102-1110413 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT105511 WP_000126294.1 NZ_CP029369:1110410-1110829 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT105511 NZ_CP029369:1110410-1110829 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5