Detailed information of TA system TA105500

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81866..82120
• Replicon: plasmid pQnrS1_035148
• Accession: NZ_CP029368
• Organism: Escherichia coli strain WCHEC035148

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: DI496_RS01345
• Protein ID: WP_015387399.1
• Coordinates: 81866..82072 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	82059..82120 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DI496_RS01310	77113..78024	-	912	WP_000440183.1	carbamate kinase	-
DI496_RS01315	78035..79255	-	1221	WP_000410951.1	arginine deiminase	-
DI496_RS01320	80148..80630	+	483	Protein_85	VENN motif pre-toxin domain-containing protein	-
DI496_RS01325	80576..81022	-	447	Protein_86	incFII family plasmid replication initiator RepA	-
DI496_RS01330	81015..81089	-	75	WP_032336874.1	RepA leader peptide Tap	-
DI496_RS01340	81325..81582	-	258	WP_000083833.1	replication regulatory protein RepA	-
DI496_RS01345	81866..82072	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	82059..82120	+	62	NuclAT_0	-	Antitoxin
-	82059..82120	+	62	NuclAT_0	-	Antitoxin
-	82059..82120	+	62	NuclAT_0	-	Antitoxin
-	82059..82120	+	62	NuclAT_0	-	Antitoxin
DI496_RS01355	82259..82441	-	183	WP_000968309.1	hypothetical protein	-
DI496_RS01365	82542..83158	+	617	Protein_91	IS1 family transposase	-
DI496_RS01370	83196..84767	-	1572	WP_023149734.1	IS66 family transposase	-
DI496_RS01375	84787..85134	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
DI496_RS01380	85134..85811	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
DI496_RS26195	85866..85955	+	90	Protein_95	IS1 family transposase	-
DI496_RS01395	86256..86468	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA14 / qnrS1 / tet(A) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..122194	122194

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T105500 WP_015387399.1 NZ_CP029368:c82072-81866 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T105500 NZ_CP029368:c82072-81866 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT105500 NZ_CP029368:82059-82120 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin