Detailed information of TA system TA105496

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29256..29520
• Replicon: plasmid pCMY42_035148
• Accession: NZ_CP029367
• Organism: Escherichia coli strain WCHEC035148

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: DI496_RS00785
• Protein ID: WP_001387489.1
• Coordinates: 29256..29408 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	29460..29520 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DI496_RS00750	24348..24881	-	534	WP_001221666.1	lipocalin family protein	-
DI496_RS00755	24975..26120	-	1146	WP_039023136.1	class C extended-spectrum beta-lactamase CMY-42	-
DI496_RS00760	26573..27270	-	698	Protein_30	IS1 family transposase	-
DI496_RS00770	27495..27704	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
DI496_RS26190	28096..28272	+	177	WP_001054897.1	hypothetical protein	-
DI496_RS00775	28337..28633	-	297	WP_011264046.1	hypothetical protein	-
DI496_RS00780	28933..29184	+	252	WP_001291964.1	hypothetical protein	-
DI496_RS00785	29256..29408	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	29460..29520	+	61	NuclAT_0	-	Antitoxin
-	29460..29520	+	61	NuclAT_0	-	Antitoxin
-	29460..29520	+	61	NuclAT_0	-	Antitoxin
-	29460..29520	+	61	NuclAT_0	-	Antitoxin
DI496_RS00790	29723..30070	+	348	Protein_36	protein finQ	-
DI496_RS00795	30256..31413	+	1158	Protein_37	IS1380-like element ISEc9 family transposase	-
DI496_RS00805	31469..32166	+	698	Protein_38	IS1 family transposase	-
DI496_RS00810	32180..32485	-	306	Protein_39	transcription termination factor NusG	-
DI496_RS00820	32927..33214	-	288	WP_000074855.1	conjugal transfer protein TraA	-
DI496_RS00825	33132..33395	-	264	WP_032180564.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..34321	34321
	-	inside	IScluster/Tn	blaCMY-42	-	24975..31744	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T105496 WP_001387489.1 NZ_CP029367:c29408-29256 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T105496 NZ_CP029367:c29408-29256 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT105496 NZ_CP029367:29460-29520 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin