Detailed information of TA system TA105495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 77748..78459
• Replicon: plasmid p1_035148
• Accession: NZ_CP029365
• Organism: Escherichia coli strain WCHEC035148

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: DI496_RS00490
• Protein ID: WP_000162415.1
• Coordinates: 78157..78459 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DI496_RS00485
• Protein ID: WP_000806445.1
• Coordinates: 77748..78086 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DI496_RS00450	72905..73132	-	228	WP_001396850.1	hypothetical protein	-
DI496_RS00455	73318..73926	-	609	WP_000776434.1	hypothetical protein	-
DI496_RS00460	74210..74863	+	654	WP_001557715.1	hypothetical protein	-
DI496_RS00465	75192..75521	+	330	WP_112031932.1	baseplate protein	-
DI496_RS00470	75514..76707	+	1194	WP_074470350.1	phage tail protein	-
DI496_RS00475	76741..77469	-	729	WP_109157909.1	hypothetical protein	-
DI496_RS00485	77748..78086	-	339	WP_000806445.1	HigA family addiction module antidote protein	Antitoxin
DI496_RS00490	78157..78459	-	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DI496_RS00495	78622..79413	+	792	WP_112031933.1	phage tail protein	-
DI496_RS00500	79410..80177	+	768	WP_000203293.1	hypothetical protein	-
DI496_RS00505	80181..81161	+	981	WP_000046500.1	hypothetical protein	-
DI496_RS00510	81158..81811	+	654	WP_087503501.1	baseplate protein	-
DI496_RS00515	81871..82776	+	906	WP_112031934.1	recombination-associated protein RdgC	-
DI496_RS00520	82760..83440	+	681	WP_000812238.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89248	89248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T105495 WP_000162415.1 NZ_CP029365:c78459-78157 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T105495 NZ_CP029365:c78459-78157 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT105495 WP_000806445.1 NZ_CP029365:c78086-77748 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT105495 NZ_CP029365:c78086-77748 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAACAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3