Detailed information of TA system TA105464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1201399..1202072
• Replicon: chromosome
• Accession: NZ_CP029332
• Organism: Mycobacterium avium subsp. hominissuis strain MAC109

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2A2ZF02
• Locus tag: DFS55_RS05820
• Protein ID: WP_033718548.1
• Coordinates: 1201725..1202072 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2A2ZDH9
• Locus tag: DFS55_RS05815
• Protein ID: WP_033718509.1
• Coordinates: 1201399..1201728 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DFS55_RS05790	1196578..1196814	-	237	Protein_1136	hypothetical protein	-
DFS55_RS25895	1196910..1197665	-	756	WP_062887723.1	IS21-like element helper ATPase IstB	-
DFS55_RS25900	1197729..1199267	-	1539	WP_062887722.1	IS21 family transposase	-
DFS55_RS05800	1199458..1200081	-	624	Protein_1139	hypothetical protein	-
DFS55_RS05805	1200146..1200598	-	453	WP_033718506.1	GNAT family N-acetyltransferase	-
DFS55_RS05810	1200595..1201197	-	603	WP_129560154.1	hypothetical protein	-
DFS55_RS05815	1201399..1201728	-	330	WP_033718509.1	helix-turn-helix transcriptional regulator	Antitoxin
DFS55_RS05820	1201725..1202072	-	348	WP_033718548.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DFS55_RS05825	1202125..1203453	-	1329	WP_116671117.1	site-specific integrase	-
DFS55_RS05830	1203450..1203638	-	189	WP_062886189.1	helix-turn-helix domain-containing protein	-
DFS55_RS05835	1203610..1204458	-	849	WP_076221054.1	helix-turn-helix domain-containing protein	-
DFS55_RS25500	1206050..1206475	-	426	WP_129560155.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1184367..1221213	36846
	-	flank	IS/Tn	-	-	1196910..1197665	755

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-94)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12988.78 Da
• Isoelectric Point: 4.3961

>T105464 WP_033718548.1 NZ_CP029332:c1202072-1201725 [Mycobacterium avium subsp. hominissuis]
MSWDVILLEEVETWYFSLDEDAMAAVTGAIDLLELEGPTLGRPTVDKVKGSKFHSMKELRPAGTSIRILFIFDPRRQAIL
LLGGDKAGNWKSWYDKNIPIAERSYEDWLATEGGE

Nucleotide

• Length: 348 bp

>T105464 NZ_CP029332:c1202072-1201725 [Mycobacterium avium subsp. hominissuis]
ATGTCCTGGGATGTGATCCTGCTGGAGGAGGTCGAAACCTGGTACTTCTCGCTCGACGAAGACGCGATGGCAGCGGTCAC
CGGGGCCATCGACCTGCTCGAGCTGGAAGGACCAACGCTAGGCCGGCCGACGGTGGACAAGGTCAAGGGATCGAAGTTCC
ACAGCATGAAGGAGCTACGGCCAGCCGGCACCAGCATCCGGATCCTGTTCATCTTCGATCCGCGGCGTCAAGCGATCCTA
TTGCTCGGCGGGGACAAGGCAGGCAACTGGAAAAGCTGGTACGACAAGAACATTCCGATAGCTGAGCGAAGCTACGAGGA
CTGGCTCGCGACTGAGGGAGGTGAATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11922.43 Da
• Isoelectric Point: 7.4050

>AT105464 WP_033718509.1 NZ_CP029332:c1201728-1201399 [Mycobacterium avium subsp. hominissuis]
MSMARNWRDIRADAIAQGRLDPARADAARNEMHDAVQAHRLAEIRKAQGHRRQADVAVLMGVSQARVSKLESGDLSHTEL
GTLQSYVAALGGSLRVVAEFDEKTVELTT

Nucleotide

• Length: 330 bp

>AT105464 NZ_CP029332:c1201728-1201399 [Mycobacterium avium subsp. hominissuis]
ATGAGCATGGCACGCAACTGGCGTGATATCCGTGCCGACGCCATCGCGCAGGGGCGCCTGGACCCCGCCCGCGCCGATGC
CGCGCGCAACGAGATGCACGACGCGGTCCAGGCCCACCGGCTCGCCGAAATTCGCAAAGCCCAAGGCCACAGACGTCAGG
CCGACGTCGCTGTCCTGATGGGGGTTTCGCAAGCGCGAGTGTCGAAGTTGGAGAGCGGCGACCTGTCGCACACCGAGCTT
GGCACGCTCCAGTCTTACGTGGCGGCGTTGGGCGGCAGCCTCCGGGTCGTCGCCGAGTTTGACGAGAAGACCGTGGAGCT
GACAACTTGA

Structures

Toxin

• AlphaFold DB: A0A2A2ZF02

Antitoxin

• AlphaFold DB: A0A2A2ZDH9