Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1058027..1058568 Replicon chromosome
Accession NZ_CP029328
Organism Escherichia coli strain A1

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag AOY52_RS04960 Protein ID WP_000615976.1
Coordinates 1058027..1058305 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AOY52_RS04965 Protein ID WP_000729704.1
Coordinates 1058308..1058568 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY52_RS04945 (1055609) 1055609..1056187 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AOY52_RS04950 (1056393) 1056393..1057160 + 768 WP_000333380.1 class II glutamine amidotransferase -
AOY52_RS04955 (1057131) 1057131..1057871 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY52_RS04960 (1058027) 1058027..1058305 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY52_RS04965 (1058308) 1058308..1058568 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY52_RS04970 (1058754) 1058754..1059527 + 774 WP_000543897.1 C40 family peptidase -
AOY52_RS04975 (1059584) 1059584..1059940 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
AOY52_RS04980 (1059933) 1059933..1060211 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AOY52_RS04985 (1060316) 1060316..1062028 - 1713 Protein_957 flagellar biosynthesis protein FlhA -
AOY52_RS04990 (1062000) 1062000..1062785 + 786 WP_000207590.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T105438 WP_000615976.1 NZ_CP029328:c1058305-1058027 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T105438 NZ_CP029328:c1058305-1058027 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT105438 WP_000729704.1 NZ_CP029328:c1058568-1058308 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT105438 NZ_CP029328:c1058568-1058308 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References