Detailed information of TA system TA105435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 157318..157920
• Replicon: chromosome
• Accession: NZ_CP029328
• Organism: Escherichia coli strain A1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY52_RS00760
• Protein ID: WP_000897305.1
• Coordinates: 157318..157629 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY52_RS00765
• Protein ID: WP_000356397.1
• Coordinates: 157630..157920 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY52_RS00730 (152348)	152348..153133	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
AOY52_RS00735 (153232)	153232..153831	+	600	WP_001295269.1	glucose-1-phosphatase	-
AOY52_RS00740 (153825)	153825..154697	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY52_RS00745 (154694)	154694..155131	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY52_RS00750 (155176)	155176..156117	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
AOY52_RS00755 (156181)	156181..157089	-	909	WP_001162704.1	alpha/beta hydrolase	-
AOY52_RS00760 (157318)	157318..157629	+	312	WP_000897305.1	hypothetical protein	Toxin
AOY52_RS00765 (157630)	157630..157920	+	291	WP_000356397.1	NadS family protein	Antitoxin
AOY52_RS00770 (158525)	158525..158743	+	219	WP_001297075.1	CopG family transcriptional regulator	-
AOY52_RS00775 (158962)	158962..159204	+	243	WP_001086388.1	protein YiiF	-
AOY52_RS00780 (159534)	159534..160463	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AOY52_RS00785 (160460)	160460..161095	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY52_RS00790 (161092)	161092..161994	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T105435 WP_000897305.1 NZ_CP029328:157318-157629 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T105435 NZ_CP029328:157318-157629 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT105435 WP_000356397.1 NZ_CP029328:157630-157920 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT105435 NZ_CP029328:157630-157920 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7