Detailed information of TA system TA105434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4245174..4245973
• Replicon: chromosome
• Accession: NZ_CP029327
• Organism: Escherichia coli strain A37

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: AOY90_RS20475
• Protein ID: WP_001592740.1
• Coordinates: 4245509..4245973 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: AOY90_RS20470
• Protein ID: WP_001296435.1
• Coordinates: 4245174..4245509 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY90_RS20455 (4240959)	4240959..4241729	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
AOY90_RS20460 (4241745)	4241745..4243079	-	1335	WP_240728661.1	galactarate/glucarate/glycerate transporter GarP	-
AOY90_RS20465 (4243454)	4243454..4245025	+	1572	WP_001273763.1	galactarate dehydratase	-
AOY90_RS20470 (4245174)	4245174..4245509	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
AOY90_RS20475 (4245509)	4245509..4245973	+	465	WP_001592740.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
AOY90_RS20480 (4246028)	4246028..4246837	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
AOY90_RS20485 (4247086)	4247086..4248366	+	1281	WP_000681941.1	tagatose-bisphosphate aldolase subunit KbaZ	-
AOY90_RS20490 (4248389)	4248389..4248862	+	474	WP_001298322.1	PTS N-acetylgalactosamine transporter subunit IIB	-
AOY90_RS20495 (4248873)	4248873..4249652	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
AOY90_RS20500 (4249642)	4249642..4250520	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
AOY90_RS20505 (4250538)	4250538..4250972	+	435	WP_000948834.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17776.15 Da
• Isoelectric Point: 9.6927

>T105434 WP_001592740.1 NZ_CP029327:4245509-4245973 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KNWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T105434 NZ_CP029327:4245509-4245973 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAAACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT105434 WP_001296435.1 NZ_CP029327:4245174-4245509 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT105434 NZ_CP029327:4245174-4245509 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: S1PPV5