Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2381757..2382310 Replicon chromosome
Accession NZ_CP029327
Organism Escherichia coli strain A37

Toxin (Protein)


Gene name higB Uniprot ID A0A0G9FUJ7
Locus tag AOY90_RS11440 Protein ID WP_000605680.1
Coordinates 2382041..2382310 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AOY90_RS11435 Protein ID WP_000781370.1
Coordinates 2381757..2382041 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY90_RS11420 (2376938) 2376938..2379985 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
AOY90_RS11425 (2379998) 2379998..2380882 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
AOY90_RS11430 (2380875) 2380875..2381528 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
AOY90_RS11435 (2381757) 2381757..2382041 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
AOY90_RS11440 (2382041) 2382041..2382310 - 270 WP_000605680.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY90_RS11445 (2382496) 2382496..2383506 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
AOY90_RS11450 (2383640) 2383640..2385337 - 1698 WP_000433462.1 malate dehydrogenase -
AOY90_RS11455 (2385494) 2385494..2385631 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AOY90_RS11460 (2385733) 2385733..2385948 - 216 WP_000495766.1 biofilm-dependent modulation protein -
AOY90_RS11465 (2386293) 2386293..2386724 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10181.73 Da        Isoelectric Point: 6.8582

>T105419 WP_000605680.1 NZ_CP029327:c2382310-2382041 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

>T105419 NZ_CP029327:c2382310-2382041 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACACGATT
ACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTACATCCGTTAA
GCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAATGGCGATGCA
TACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT105419 WP_000781370.1 NZ_CP029327:c2382041-2381757 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT105419 NZ_CP029327:c2382041-2381757 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FUJ7

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References