Detailed information of TA system TA105407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 166703..167305
• Replicon: chromosome
• Accession: NZ_CP029327
• Organism: Escherichia coli strain A37

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AOY90_RS00785
• Protein ID: WP_000897302.1
• Coordinates: 166703..167014 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY90_RS00790
• Protein ID: WP_000356397.1
• Coordinates: 167015..167305 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY90_RS00755 (161734)	161734..162519	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
AOY90_RS00760 (162618)	162618..163217	+	600	WP_001298594.1	glucose-1-phosphatase	-
AOY90_RS00765 (163211)	163211..164083	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY90_RS00770 (164080)	164080..164517	+	438	WP_000560976.1	D-aminoacyl-tRNA deacylase	-
AOY90_RS00775 (164562)	164562..165503	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY90_RS00780 (165566)	165566..166474	-	909	WP_001357065.1	alpha/beta hydrolase	-
AOY90_RS00785 (166703)	166703..167014	+	312	WP_000897302.1	hypothetical protein	Toxin
AOY90_RS00790 (167015)	167015..167305	+	291	WP_000356397.1	NadS family protein	Antitoxin
AOY90_RS00795 (167664)	167664..167942	+	279	WP_001296612.1	hypothetical protein	-
AOY90_RS00800 (168349)	168349..168567	+	219	WP_001314326.1	CopG family transcriptional regulator	-
AOY90_RS00805 (168783)	168783..169712	-	930	WP_240728701.1	formate dehydrogenase accessory protein FdhE	-
AOY90_RS00810 (169709)	169709..170344	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY90_RS00815 (170341)	170341..171243	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T105407 WP_000897302.1 NZ_CP029327:166703-167014 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T105407 NZ_CP029327:166703-167014 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT105407 WP_000356397.1 NZ_CP029327:167015-167305 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT105407 NZ_CP029327:167015-167305 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416