Detailed information of TA system TA105364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-YefM
• Location: 2659222..2659737
• Replicon: chromosome
• Accession: NZ_CP029256
• Organism: Christensenella minuta strain DSM 22607

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: B1H56_RS12670
• Protein ID: WP_066521146.1
• Coordinates: 2659222..2659482 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A136Q997
• Locus tag: B1H56_RS12675
• Protein ID: WP_066521150.1
• Coordinates: 2659486..2659737 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1H56_RS12650	2654888..2655307	+	420	WP_066521127.1	hypothetical protein	-
B1H56_RS12655	2655315..2656127	+	813	WP_066739797.1	class B sortase	-
B1H56_RS12660	2656124..2657014	+	891	WP_066521129.1	1-phosphofructokinase	-
B1H56_RS14670	2657585..2657761	+	177	WP_156468701.1	hypothetical protein	-
B1H56_RS12665	2657969..2659225	-	1257	WP_066521143.1	tyrosine-type recombinase/integrase	-
B1H56_RS12670	2659222..2659482	-	261	WP_066521146.1	Txe/YoeB family addiction module toxin	Toxin
B1H56_RS12675	2659486..2659737	-	252	WP_066521150.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
B1H56_RS12680	2659799..2660338	-	540	WP_066521152.1	hypothetical protein	-
B1H56_RS12685	2660389..2660604	-	216	WP_066521154.1	hypothetical protein	-
B1H56_RS12690	2660641..2660994	-	354	WP_066521156.1	helix-turn-helix domain-containing protein	-
B1H56_RS12695	2661425..2661646	+	222	WP_066521164.1	hypothetical protein	-
B1H56_RS12700	2661646..2661897	+	252	WP_066521167.1	helix-turn-helix transcriptional regulator	-
B1H56_RS12705	2661907..2662239	+	333	WP_066521170.1	hypothetical protein	-
B1H56_RS12710	2662236..2662574	+	339	WP_066521173.1	hypothetical protein	-
B1H56_RS12715	2662668..2662853	+	186	WP_066521177.1	hypothetical protein	-
B1H56_RS12720	2662850..2663077	+	228	WP_066521179.1	hypothetical protein	-
B1H56_RS12725	2663070..2663318	+	249	WP_066521180.1	hypothetical protein	-
B1H56_RS12730	2663321..2664085	+	765	WP_121419011.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2657969..2721626	63657

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (7-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10098.75 Da
• Isoelectric Point: 10.0980

>T105364 WP_066521146.1 NZ_CP029256:c2659482-2659222 [Christensenella minuta]
MYDVQLTKRAFKDYAKLKQAGILKKAGELINILKQNPFQAPPPYEKLVGDLQGYYSRRINVQHRLVYSVDKDAQIVVIRS
MWTHYE

Nucleotide

• Length: 261 bp

>T105364 NZ_CP029256:c2659482-2659222 [Christensenella minuta]
ATGTACGATGTGCAATTGACAAAACGAGCCTTCAAGGATTATGCAAAATTGAAACAGGCGGGTATACTGAAAAAGGCCGG
AGAGCTAATTAATATTTTAAAGCAAAATCCCTTCCAGGCTCCCCCGCCTTACGAAAAGCTTGTAGGCGACTTGCAAGGCT
ATTACAGCCGTCGTATCAACGTACAGCATAGATTGGTCTACTCGGTGGATAAGGATGCTCAAATCGTCGTTATACGCAGC
ATGTGGACGCATTACGAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9436.49 Da
• Isoelectric Point: 3.9541

>AT105364 WP_066521150.1 NZ_CP029256:c2659737-2659486 [Christensenella minuta]
MEAINITKARQNLYKLVKDTNESHAPIQILGKDGNAVLLSEDDWRSIQETLYLYSVPGLVESIKESENEPVEEMVSADDL
DWE

Nucleotide

• Length: 252 bp

>AT105364 NZ_CP029256:c2659737-2659486 [Christensenella minuta]
ATGGAGGCGATCAATATTACAAAAGCACGGCAGAATTTATATAAGCTTGTGAAAGATACCAACGAATCCCACGCCCCAAT
CCAGATACTCGGCAAGGATGGAAACGCTGTTCTTCTCTCCGAGGACGATTGGAGGTCTATACAGGAGACTTTATACCTGT
ATTCTGTTCCTGGCCTTGTAGAGAGCATCAAGGAGAGCGAGAACGAGCCTGTAGAAGAAATGGTAAGCGCTGATGACCTC
GATTGGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A136Q997