Detailed information of TA system TA105337

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 55985..56411
• Replicon: plasmid pTB201
• Accession: NZ_CP029243
• Organism: Escherichia coli strain ECCRA-119

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C7235_RS25675
• Protein ID: WP_001312861.1
• Coordinates: 55985..56143 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	56187..56411 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7235_RS25630	51032..51259	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
C7235_RS25635	51353..52039	-	687	WP_000332484.1	PAS domain-containing protein	-
C7235_RS25640	52230..52613	-	384	WP_000124981.1	relaxosome protein TraM	-
C7235_RS25645	52890..53537	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
C7235_RS25650	53834..54655	-	822	WP_001234469.1	DUF945 domain-containing protein	-
C7235_RS25655	54776..55063	-	288	WP_000107535.1	hypothetical protein	-
C7235_RS27940	55088..55294	-	207	WP_000547939.1	hypothetical protein	-
C7235_RS25675	55985..56143	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	56187..56411	-	225	NuclAT_0	-	Antitoxin
-	56187..56411	-	225	NuclAT_0	-	Antitoxin
-	56187..56411	-	225	NuclAT_0	-	Antitoxin
-	56187..56411	-	225	NuclAT_0	-	Antitoxin
C7235_RS27725	56223..56411	+	189	WP_001299721.1	hypothetical protein	-
C7235_RS25680	56423..57142	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
C7235_RS25685	57139..57573	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
C7235_RS25690	57628..59586	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
C7235_RS25695	59645..59878	-	234	WP_000006004.1	DUF905 domain-containing protein	-
C7235_RS25700	59934..60461	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
C7235_RS25720	60934..61206	-	273	WP_072133095.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / aadA2 / dfrA12 / sitABCD	iroB / iroC / iroD / iroE / iroN	1..146268	146268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T105337 WP_001312861.1 NZ_CP029243:c56143-55985 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T105337 NZ_CP029243:c56143-55985 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT105337 NZ_CP029243:c56411-56187 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin