Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1852331..1852833 Replicon chromosome
Accession NZ_CP029242
Organism Escherichia coli strain ECCRA-119

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C7235_RS09500 Protein ID WP_000767829.1
Coordinates 1852579..1852833 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A2S1Y4D6
Locus tag C7235_RS09495 Protein ID WP_001259254.1
Coordinates 1852331..1852582 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7235_RS09465 1847509..1848576 - 1068 WP_000080109.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C7235_RS09470 1848576..1849646 - 1071 WP_072645751.1 histidinol-phosphate transaminase -
C7235_RS09475 1849643..1850947 - 1305 WP_001345878.1 histidinol dehydrogenase -
C7235_RS09480 1850953..1851852 - 900 WP_072645750.1 ATP phosphoribosyltransferase -
C7235_RS09490 1851998..1852048 - 51 WP_001364200.1 his operon leader peptide -
C7235_RS09495 1852331..1852582 + 252 WP_001259254.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C7235_RS09500 1852579..1852833 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C7235_RS09505 1852916..1853740 + 825 WP_000754737.1 SDR family oxidoreductase -
C7235_RS09510 1853786..1854715 + 930 WP_000803351.1 LysR family transcriptional regulator -
C7235_RS09515 1854930..1854992 + 63 WP_010723108.1 membrane protein YoeI -
C7235_RS09520 1854982..1856340 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C7235_RS09525 1856519..1857577 + 1059 WP_024193414.1 transport protein YeeE -
C7235_RS09530 1857591..1857818 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T105319 WP_000767829.1 NZ_CP029242:1852579-1852833 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T105319 NZ_CP029242:1852579-1852833 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9292.57 Da        Isoelectric Point: 4.5822

>AT105319 WP_001259254.1 NZ_CP029242:1852331-1852582 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGIGTEKD
IIE

Download         Length: 252 bp

>AT105319 NZ_CP029242:1852331-1852582 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCATAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A2S1Y4D6

References