105278

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 254118..254812 Replicon chromosome
Accession NZ_CP029239
Organism Escherichia coli strain BH212

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag DF195_RS01200 Protein ID WP_001263489.1
Coordinates 254414..254812 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag DF195_RS01195 Protein ID WP_000554758.1
Coordinates 254118..254411 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DF195_RS01175 249750..250247 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
DF195_RS01180 250471..252183 - 1713 Protein_229 flagellar biosynthesis protein FlhA -
DF195_RS01185 252155..252940 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
DF195_RS01190 253011..254066 + 1056 WP_001226164.1 DNA polymerase IV -
DF195_RS01195 254118..254411 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
DF195_RS01200 254414..254812 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
DF195_RS01205 254822..255274 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
DF195_RS01210 255592..255798 + 207 Protein_235 RtcB family protein -
DF195_RS01215 255794..256315 + 522 Protein_236 peptide chain release factor H -
DF195_RS01220 256372..257829 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
DF195_RS01225 258090..258548 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 259144..259224 + 81 NuclAT_11 - -
- 259144..259224 + 81 NuclAT_11 - -
- 259144..259224 + 81 NuclAT_11 - -
- 259144..259224 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T105278 WP_001263489.1 NZ_CP029239:254414-254812 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T105278 NZ_CP029239:254414-254812 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT105278 WP_000554758.1 NZ_CP029239:254118-254411 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT105278 NZ_CP029239:254118-254411 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References