Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2089211..2089713 Replicon chromosome
Accession NZ_CP029238
Organism Escherichia coli strain CJ236

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DF192_RS10435 Protein ID WP_000767829.1
Coordinates 2089211..2089465 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DF192_RS10440 Protein ID WP_001259255.1
Coordinates 2089462..2089713 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DF192_RS10405 2084226..2084453 - 228 WP_000234896.1 sulfurtransferase TusA -
DF192_RS10410 2084467..2085525 - 1059 WP_000492339.1 transport protein YeeE -
DF192_RS10415 2085704..2087062 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DF192_RS10420 2087052..2087114 - 63 WP_010723108.1 membrane protein YoeI -
DF192_RS10425 2087329..2088258 - 930 WP_000803351.1 LysR family transcriptional regulator -
DF192_RS10430 2088304..2089128 - 825 WP_000754737.1 SDR family oxidoreductase -
DF192_RS10435 2089211..2089465 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DF192_RS10440 2089462..2089713 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DF192_RS10445 2089996..2090046 + 51 WP_001364200.1 his operon leader peptide -
DF192_RS10450 2090192..2091091 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DF192_RS10455 2091097..2092401 + 1305 WP_000009594.1 histidinol dehydrogenase -
DF192_RS10460 2092398..2093468 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
DF192_RS10465 2093468..2094535 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T105260 WP_000767829.1 NZ_CP029238:c2089465-2089211 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T105260 NZ_CP029238:c2089465-2089211 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT105260 WP_001259255.1 NZ_CP029238:c2089713-2089462 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT105260 NZ_CP029238:c2089713-2089462 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References