Detailed information of TA system TA105224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-couple_hipB
• Location: 1403877..1404525
• Replicon: chromosome
• Accession: NZ_CP029207
• Organism: Streptococcus constellatus strain TCV107

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F9P6Y3
• Locus tag: DEH83_RS07200
• Protein ID: WP_006268450.1
• Coordinates: 1403877..1404242 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DEH83_RS07205
• Protein ID: WP_150890982.1
• Coordinates: 1404232..1404525 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DEH83_RS07160 (DEH83_07170)	1399296..1400123	+	828	WP_255776480.1	ATP-binding protein	-
DEH83_RS07165 (DEH83_07175)	1400424..1400915	+	492	WP_255776481.1	hypothetical protein	-
DEH83_RS07170	1401235..1401411	+	177	WP_006267997.1	hypothetical protein	-
DEH83_RS07175 (DEH83_07180)	1401491..1402123	+	633	WP_255776482.1	hypothetical protein	-
DEH83_RS07180 (DEH83_07185)	1402124..1402603	+	480	WP_255776483.1	hypothetical protein	-
DEH83_RS07185 (DEH83_07190)	1402584..1402994	+	411	WP_255776484.1	hypothetical protein	-
DEH83_RS07190 (DEH83_07195)	1403277..1403456	+	180	WP_255776485.1	hypothetical protein	-
DEH83_RS07195 (DEH83_07200)	1403536..1403811	+	276	WP_255776486.1	terminase small subunit	-
DEH83_RS07200 (DEH83_07205)	1403877..1404242	+	366	WP_006268450.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DEH83_RS07205 (DEH83_07210)	1404232..1404525	+	294	WP_150890982.1	helix-turn-helix transcriptional regulator	Antitoxin
DEH83_RS07210 (DEH83_07215)	1404617..1405162	+	546	WP_255776487.1	hypothetical protein	-
DEH83_RS07215 (DEH83_07220)	1405511..1407577	+	2067	WP_255776488.1	penicillin-binding protein PBP2B	-
DEH83_RS07220 (DEH83_07225)	1407588..1408184	+	597	WP_039677306.1	recombination mediator RecR	-
DEH83_RS07225 (DEH83_07230)	1408315..1409361	+	1047	WP_049481403.1	D-alanine--D-alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1393257..1408184	14927

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (35-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-88)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14448.63 Da
• Isoelectric Point: 9.7920

>T105224 WP_006268450.1 NZ_CP029207:1403877-1404242 [Streptococcus constellatus]
VHNIYFYKDKNGNEPILDYMRELASKKGKDSRIKLNKINDYIELLSQHGTRTGEPYIKHLDAEIWELRPLRDRILFVAWI
DGSFVLLHHFMKKTQKTPKREIEQAKRELADLKERGLDNEK

Nucleotide

• Length: 366 bp

>T105224 NZ_CP029207:1403877-1404242 [Streptococcus constellatus]
GTGCATAATATCTATTTTTACAAGGATAAAAACGGTAATGAGCCTATTTTAGATTATATGCGAGAGCTTGCCAGTAAAAA
GGGAAAAGATAGTAGAATTAAACTCAATAAAATCAATGATTATATTGAGTTGTTAAGTCAACACGGAACACGCACAGGCG
AGCCATATATTAAGCATTTAGATGCTGAAATTTGGGAACTAAGACCATTAAGGGATAGAATTTTATTTGTTGCATGGATT
GATGGTAGCTTTGTTTTACTCCATCATTTCATGAAAAAGACACAGAAAACACCAAAAAGAGAGATTGAACAAGCTAAACG
TGAGTTAGCAGACTTAAAAGAAAGAGGTTTAGACAATGAAAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10811.48 Da
• Isoelectric Point: 4.9123

>AT105224 WP_150890982.1 NZ_CP029207:1404232-1404525 [Streptococcus constellatus]
MKNSAIGSNWKDVRAELFSKEEILESDMRVAIMSELIEARNERGISQKKLEELSGVSQPVIARMETGKTSPQLDTVLKVL
ASLDKTLAVVPLEREQV

Nucleotide

• Length: 294 bp

>AT105224 NZ_CP029207:1404232-1404525 [Streptococcus constellatus]
ATGAAAAATAGTGCAATTGGTAGTAACTGGAAAGATGTAAGAGCTGAGTTATTCAGCAAAGAAGAAATTTTAGAAAGTGA
TATGCGCGTGGCTATCATGAGTGAGCTTATCGAGGCTAGGAATGAAAGGGGCATTAGTCAAAAAAAACTAGAGGAACTGA
GTGGCGTTAGTCAGCCAGTCATAGCTAGAATGGAGACAGGAAAAACAAGCCCACAGCTTGATACAGTATTAAAGGTTTTG
GCAAGTCTTGATAAGACTTTAGCAGTTGTACCACTAGAGCGTGAACAAGTCTAG

Structures

Toxin

• AlphaFold DB: F9P6Y3