Detailed information of TA system TA105218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 487603..488963
• Replicon: chromosome
• Accession: NZ_CP029206
• Organism: Actinobacillus porcitonsillarum strain 9953L55

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: DDU33_RS02470
• Protein ID: WP_108922912.1
• Coordinates: 487920..488963 (+)
• Length: 348 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A2U8FHI1
• Locus tag: DDU33_RS02465
• Protein ID: WP_005820436.1
• Coordinates: 487603..487920 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDU33_RS02440	482707..483174	-	468	WP_005820444.1	23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH	-
DDU33_RS02445	483174..483488	-	315	WP_108922906.1	ribosome silencing factor	-
DDU33_RS02450	483774..486368	-	2595	WP_108922908.1	DNA mismatch repair protein MutS	-
DDU33_RS02455	486482..487111	+	630	WP_108922910.1	glutathione S-transferase family protein	-
DDU33_RS02460	487307..487615	+	309	WP_005820437.1	helix-turn-helix transcriptional regulator	-
DDU33_RS02465	487603..487920	+	318	WP_005820436.1	HipA N-terminal domain-containing protein	Antitoxin
DDU33_RS02470	487920..488963	+	1044	WP_108922912.1	HipA domain-containing protein	Toxin
DDU33_RS02475	489047..490033	-	987	WP_108922914.1	transposase	-
DDU33_RS02480	490483..491046	-	564	WP_108922916.1	DUF5358 domain-containing protein	-
DDU33_RS02485	491099..492397	-	1299	WP_108922918.1	hypothetical protein	-
DDU33_RS02490	492410..492880	-	471	WP_108922920.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-293)

Antitoxin

• HipA, N-terminal subdomain 1 (9-101)

Sequences

Toxin

Protein

• Length: 348 a.a.
• Molecular weight: 39776.58 Da
• Isoelectric Point: 6.3173

>T105218 WP_108922912.1 NZ_CP029206:487920-488963 [Actinobacillus porcitonsillarum]
MSVCRITLQALTNSEEKTGYSTKGLLALTGNEKFNPMLPFSRQEFTFSTPQKQKGMSISGYQPKLQLIIQDNQFTSIDQQ
GLYILKPSPADYPNLAENEHATMQLMKKLGFCVPENGLIAFNASELTAEKEFAFVIKRFDRTEEGKPIHKEQLDGAMNVR
EKYGKIGNDNEQYISYEQAVKFILTHTENNLSQRQELFRRIVYAYLLGNNDLHLRNFSLIHSKEGTITLAPIYDFVSVAP
YSELFHSSILALPLLAKEEGGKELAHGFDTQYGEYIGQDFIEFGENIGLSKRLIIEKLLPEIKDEAKMVEQVYQTSFLPE
KHKEQILKAYLRRLNLLFILNEEKLLK

Nucleotide

• Length: 1044 bp

>T105218 NZ_CP029206:487920-488963 [Actinobacillus porcitonsillarum]
ATGTCCGTTTGTCGAATTACATTACAAGCACTCACAAACAGTGAAGAAAAAACAGGTTATAGCACAAAGGGGCTTTTGGC
TTTAACCGGCAATGAAAAATTTAATCCAATGCTTCCATTTAGCCGGCAAGAATTCACTTTCTCTACGCCTCAAAAGCAAA
AAGGCATGAGTATTTCAGGTTATCAACCCAAACTACAACTGATTATTCAAGATAACCAGTTTACAAGCATTGATCAGCAA
GGATTATATATTCTCAAACCCTCTCCGGCGGATTACCCAAATTTGGCGGAAAATGAACACGCCACGATGCAATTAATGAA
AAAATTAGGTTTTTGTGTGCCTGAAAATGGCTTGATTGCTTTTAATGCAAGTGAATTAACCGCAGAAAAAGAATTTGCTT
TTGTGATTAAACGTTTTGATCGTACCGAAGAAGGCAAGCCCATTCATAAAGAGCAACTTGATGGTGCAATGAATGTCCGT
GAAAAATATGGGAAAATTGGCAACGACAACGAGCAATATATTAGCTATGAACAAGCGGTAAAATTTATTCTTACTCATAC
CGAAAACAATTTAAGCCAAAGGCAAGAATTATTTAGACGAATCGTTTATGCCTATCTTCTTGGAAATAATGACTTACATC
TACGGAACTTCTCGCTAATTCATTCAAAAGAAGGAACAATTACACTTGCACCTATTTATGATTTTGTTTCCGTAGCCCCT
TATTCTGAACTATTTCATAGCTCAATTTTAGCTTTACCACTATTAGCAAAAGAGGAGGGGGGAAAAGAATTGGCTCACGG
TTTCGATACACAATATGGCGAATATATCGGGCAAGATTTTATTGAATTTGGAGAGAATATCGGACTAAGTAAAAGGCTGA
TTATCGAAAAACTTCTTCCAGAAATAAAGGATGAAGCTAAAATGGTAGAACAAGTGTATCAGACCTCTTTTTTACCCGAA
AAACATAAAGAACAGATATTAAAGGCATATTTAAGAAGGCTTAACTTGCTTTTTATTTTAAACGAGGAGAAATTACTTAA
ATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12170.82 Da
• Isoelectric Point: 5.5252

>AT105218 WP_005820436.1 NZ_CP029206:487603-487920 [Actinobacillus porcitonsillarum]
MHALKRIGNVYLYEELIGQLVQDSQGFHFYYDENYQGISISLSLPISQRAFHSEHLFPYFASLVPEGWLKERYSTLQKID
ENDLFGLLLNNGKNLLGATQIFEVK

Nucleotide

• Length: 318 bp

>AT105218 NZ_CP029206:487603-487920 [Actinobacillus porcitonsillarum]
ATGCACGCTCTAAAACGTATTGGTAATGTCTATTTATATGAAGAACTAATCGGGCAACTCGTACAAGATTCACAAGGTTT
TCATTTTTATTATGATGAAAATTATCAGGGAATTTCGATTTCGCTTAGCCTTCCAATTTCACAGCGAGCGTTTCATTCTG
AGCATCTTTTCCCTTATTTTGCTTCACTTGTCCCCGAGGGTTGGTTAAAGGAACGTTATTCTACCTTGCAGAAGATTGAT
GAAAATGATCTCTTCGGATTACTCCTAAATAATGGAAAAAATTTACTTGGCGCCACACAAATTTTTGAGGTGAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2U8FHI1