Detailed information of TA system TA105217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 172078..172724
• Replicon: chromosome
• Accession: NZ_CP029206
• Organism: Actinobacillus porcitonsillarum strain 9953L55

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DDU33_RS00875
• Protein ID: WP_208620300.1
• Coordinates: 172078..172428 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: C5RZ53
• Locus tag: DDU33_RS00880
• Protein ID: WP_005818050.1
• Coordinates: 172425..172724 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDU33_RS00860	167629..169050	+	1422	WP_108922534.1	sucrose-specific PTS transporter subunit IIBC	-
DDU33_RS00865	169115..170362	+	1248	WP_108922536.1	paraquat-inducible protein A	-
DDU33_RS00870	170461..171996	+	1536	WP_108922538.1	YifB family Mg chelatase-like AAA ATPase	-
DDU33_RS00875	172078..172428	+	351	WP_208620300.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DDU33_RS00880	172425..172724	+	300	WP_005818050.1	helix-turn-helix transcriptional regulator	Antitoxin
DDU33_RS00885	172739..173521	-	783	WP_108922543.1	2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase	-
DDU33_RS00890	173521..175227	-	1707	WP_108922545.1	2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase	-
DDU33_RS00895	175413..176441	+	1029	WP_108922547.1	tryptophan--tRNA ligase	-
DDU33_RS00900	176490..177605	-	1116	WP_108922550.1	molybdenum cofactor guanylyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13665.63 Da
• Isoelectric Point: 8.9624

>T105217 WP_208620300.1 NZ_CP029206:172078-172428 [Actinobacillus porcitonsillarum]
IWTVITTDCFNKWYEQQDEDTQDSVLAHLLRLQQIGPYLARPYADTINGSAYSNMKELRIQHRGKPIRAFFAFDPLRQAI
VLCAGDKSNDKKFYEKMLRIAETEFTRYLNKLESKK

Nucleotide

• Length: 351 bp

>T105217 NZ_CP029206:172078-172428 [Actinobacillus porcitonsillarum]
ATTTGGACAGTTATTACTACTGACTGTTTTAATAAATGGTATGAACAACAGGATGAAGATACTCAAGATAGTGTACTTGC
TCATTTATTAAGATTACAACAAATCGGTCCATATTTGGCTAGACCTTATGCAGATACAATTAACGGCTCTGCTTATTCCA
ATATGAAAGAGCTTAGAATTCAACATCGCGGTAAACCTATTCGTGCATTTTTTGCTTTTGATCCATTACGTCAAGCTATC
GTACTATGCGCTGGGGATAAAAGTAATGATAAAAAATTCTATGAGAAAATGCTCCGTATAGCAGAAACCGAATTTACCCG
TTATTTAAATAAGTTGGAGAGTAAAAAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11159.01 Da
• Isoelectric Point: 7.0164

>AT105217 WP_005818050.1 NZ_CP029206:172425-172724 [Actinobacillus porcitonsillarum]
MKTLEQLMEEIPSERQAKVKALSEQLIQEVGLAHLREELNISQKQLAEALGISQPAVAKIEQKDNDPRLSTLKRYIEAMG
GKLSLTVQMPMGDSRIFRI

Nucleotide

• Length: 300 bp

>AT105217 NZ_CP029206:172425-172724 [Actinobacillus porcitonsillarum]
ATGAAAACTTTAGAACAATTAATGGAGGAAATTCCCTCAGAAAGACAAGCTAAAGTAAAAGCGTTAAGTGAACAGCTGAT
TCAGGAAGTTGGCTTAGCTCATTTAAGGGAAGAATTGAATATTTCTCAAAAACAGCTTGCAGAAGCTTTAGGTATATCTC
AGCCGGCAGTAGCTAAAATAGAACAGAAAGATAATGATCCACGCCTCTCTACACTTAAACGTTATATTGAGGCAATGGGT
GGAAAATTAAGCCTTACCGTACAAATGCCTATGGGGGATAGCCGAATTTTTAGAATTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2U8FGN7