Detailed information of TA system TA105169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4002589..4003283
• Replicon: chromosome
• Accession: NZ_CP029180
• Organism: Escherichia coli strain H9Ecoli

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: DEF50_RS21125
• Protein ID: WP_001263493.1
• Coordinates: 4002885..4003283 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: DEF50_RS21120
• Protein ID: WP_000554757.1
• Coordinates: 4002589..4002882 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DEF50_RS21100	3998221..3998718	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
DEF50_RS21105	3998942..4000654	-	1713	Protein_3831	flagellar biosynthesis protein FlhA	-
DEF50_RS21110	4000626..4001411	+	786	WP_072645198.1	putative lateral flagellar export/assembly protein LafU	-
DEF50_RS21115	4001482..4002537	+	1056	WP_001226164.1	DNA polymerase IV	-
DEF50_RS21120	4002589..4002882	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
DEF50_RS21125	4002885..4003283	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
DEF50_RS21130	4003293..4003745	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
DEF50_RS21135	4003991..4004194	+	204	Protein_3837	RNA ligase RtcB family protein	-
DEF50_RS21140	4004193..4004714	+	522	Protein_3838	peptide chain release factor H	-
DEF50_RS21145	4004771..4006228	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
DEF50_RS21150	4006489..4006947	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
DEF50_RS21155	4007039..4008283	+	1245	WP_000189541.1	esterase FrsA	-
-	4007543..4007623	+	81	NuclAT_11	-	-
-	4007543..4007623	+	81	NuclAT_11	-	-
-	4007543..4007623	+	81	NuclAT_11	-	-
-	4007543..4007623	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T105169 WP_001263493.1 NZ_CP029180:4002885-4003283 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T105169 NZ_CP029180:4002885-4003283 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT105169 WP_000554757.1 NZ_CP029180:4002589-4002882 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT105169 NZ_CP029180:4002589-4002882 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6