Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 109515..110017 Replicon chromosome
Accession NZ_CP029180
Organism Escherichia coli strain H9Ecoli

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DEF50_RS00530 Protein ID WP_000767829.1
Coordinates 109763..110017 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DEF50_RS00525 Protein ID WP_001259255.1
Coordinates 109515..109766 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DEF50_RS00495 104693..105760 - 1068 WP_000080142.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
DEF50_RS00500 105760..106830 - 1071 WP_001099220.1 histidinol-phosphate transaminase -
DEF50_RS00505 106827..108131 - 1305 WP_001355813.1 histidinol dehydrogenase -
DEF50_RS00510 108137..109036 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
DEF50_RS00520 109182..109232 - 51 WP_001364200.1 his operon leader peptide -
DEF50_RS00525 109515..109766 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DEF50_RS00530 109763..110017 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DEF50_RS00535 110100..110924 + 825 WP_001567684.1 SDR family oxidoreductase -
DEF50_RS00540 110970..111899 + 930 WP_000803360.1 LysR family transcriptional regulator -
DEF50_RS00545 112114..112176 + 63 WP_010723108.1 membrane protein YoeI -
DEF50_RS00550 112166..113524 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DEF50_RS00555 113703..114761 + 1059 WP_072645364.1 transport protein YeeE -
DEF50_RS00560 114775..115002 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T105151 WP_000767829.1 NZ_CP029180:109763-110017 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T105151 NZ_CP029180:109763-110017 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT105151 WP_001259255.1 NZ_CP029180:109515-109766 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT105151 NZ_CP029180:109515-109766 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References