Detailed information of TA system TA105103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 4347808..4348487
• Replicon: chromosome
• Accession: NZ_CP029164
• Organism: Escherichia coli strain 104

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1PK60
• Locus tag: DDV95_RS22505
• Protein ID: WP_000057523.1
• Coordinates: 4348185..4348487 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: DDV95_RS22500
• Protein ID: WP_000806442.1
• Coordinates: 4347808..4348149 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDV95_RS22490	4344051..4344983	-	933	WP_000883024.1	glutaminase A	-
DDV95_RS22495	4345246..4347750	+	2505	WP_000083999.1	copper-exporting P-type ATPase CopA	-
DDV95_RS22500	4347808..4348149	-	342	WP_000806442.1	HigA family addiction module antidote protein	Antitoxin
DDV95_RS22505	4348185..4348487	-	303	WP_000057523.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DDV95_RS22510	4348620..4349414	+	795	WP_000365161.1	TraB/GumN family protein	-
DDV95_RS22520	4349618..4350097	+	480	WP_000186638.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
DDV95_RS22525	4350134..4351786	-	1653	WP_086525247.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
DDV95_RS22535	4352004..4353224	+	1221	WP_001251634.1	fosmidomycin MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11809.44 Da
• Isoelectric Point: 10.3980

>T105103 WP_000057523.1 NZ_CP029164:c4348487-4348185 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T105103 NZ_CP029164:c4348487-4348185 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT105103 WP_000806442.1 NZ_CP029164:c4348149-4347808 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT105103 NZ_CP029164:c4348149-4347808 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: S1PK60

Antitoxin

• AlphaFold DB: S1QAY3