Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2762720..2763222 Replicon chromosome
Accession NZ_CP029164
Organism Escherichia coli strain 104

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DDV95_RS13995 Protein ID WP_000767829.1
Coordinates 2762968..2763222 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DDV95_RS13990 Protein ID WP_001259255.1
Coordinates 2762720..2762971 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DDV95_RS13960 2757892..2758959 - 1068 WP_000080097.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
DDV95_RS13965 2758959..2760029 - 1071 WP_000108970.1 histidinol-phosphate transaminase -
DDV95_RS13970 2760026..2761330 - 1305 WP_072686623.1 histidinol dehydrogenase -
DDV95_RS13975 2761336..2762235 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
DDV95_RS13985 2762387..2762437 - 51 WP_001364200.1 his operon leader peptide -
DDV95_RS13990 2762720..2762971 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DDV95_RS13995 2762968..2763222 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DDV95_RS14000 2763305..2764129 + 825 WP_075826841.1 SDR family oxidoreductase -
DDV95_RS14005 2764175..2765104 + 930 WP_001461145.1 LysR family transcriptional regulator -
DDV95_RS14010 2765319..2765381 + 63 WP_010723108.1 membrane protein YoeI -
DDV95_RS14015 2765371..2766729 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DDV95_RS14020 2766908..2767966 + 1059 WP_000492315.1 transport protein YeeE -
DDV95_RS14025 2767980..2768207 + 228 WP_050867712.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T105090 WP_000767829.1 NZ_CP029164:2762968-2763222 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T105090 NZ_CP029164:2762968-2763222 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT105090 WP_001259255.1 NZ_CP029164:2762720-2762971 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT105090 NZ_CP029164:2762720-2762971 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References