Detailed information of TA system TA105050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1101380..1101941
• Replicon: chromosome
• Accession: NZ_CP029150
• Organism: Glaesserella parasuis strain GZ20170512

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U4SMA9
• Locus tag: C3363_RS05330
• Protein ID: WP_010786777.1
• Coordinates: 1101663..1101941 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: C3363_RS05325
• Protein ID: WP_005712616.1
• Coordinates: 1101380..1101652 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3363_RS05305	1097744..1098286	+	543	WP_010786780.1	single-stranded DNA-binding protein	-
C3363_RS05310	1098492..1099706	+	1215	WP_010786779.1	ATP-binding protein	-
C3363_RS05315	1099778..1100086	-	309	Protein_1024	DUF853 family protein	-
C3363_RS05320	1100096..1101316	-	1221	WP_005712618.1	ATP-dependent RNA helicase RhlB	-
C3363_RS05325	1101380..1101652	-	273	WP_005712616.1	HigA family addiction module antidote protein	Antitoxin
C3363_RS05330	1101663..1101941	-	279	WP_010786777.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3363_RS05335	1102088..1103098	+	1011	WP_005712612.1	ornithine carbamoyltransferase	-
C3363_RS05340	1103445..1104707	+	1263	WP_075620774.1	serine hydroxymethyltransferase	-
C3363_RS05345	1104821..1106149	+	1329	WP_010786775.1	exodeoxyribonuclease VII large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10278.66 Da
• Isoelectric Point: 10.0505

>T105050 WP_010786777.1 NZ_CP029150:c1101941-1101663 [Glaesserella parasuis]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQGNLAGHWSIKVSGNWRLTFKVQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T105050 NZ_CP029150:c1101941-1101663 [Glaesserella parasuis]
ATGATTATTTCATTTAAGCATAAAGGATTGAAATTATTTTTTGAAACAGGATCAACCGCAGGTATTCAAGCAAATCACGC
TAGTAAACTTGCTTTTCAATTAGCCACTCTAAACCAAGCAAAAAGTGCTTTAGATATGAATGCACCTAGCTGGAATTTAC
ATCAATTACAAGGTAATTTAGCTGGGCATTGGTCTATCAAGGTAAGTGGAAATTGGCGTTTAACTTTTAAAGTTCAAAAT
GGTCACGCTGAAGTCGTGGACTATCAAGATTATCATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT105050 WP_005712616.1 NZ_CP029150:c1101652-1101380 [Glaesserella parasuis]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT105050 NZ_CP029150:c1101652-1101380 [Glaesserella parasuis]
ATGAAAATGTACAATCCAGCTCACCCAGGGCTTATTTTAAGAGAATATATTGAAGGATATAATGTCACAGAAGTGGCTAA
ACGTTTAGGTGTCACTCGTGTCACGCTCTCTCGTATTTTAAATGGCAAAGCCGCAATTTCACCTGAAATGGCTCTTAGAT
TAAGCCAATTATTAAAAACTAGCCCAGAATTATGGCTTGGTTTACAATCCCAATACGATCTTTGGCAGCTTGAACAACGC
CCAAGTTTTAATATTCAGCCGTTATTTGCATAG

Structures

Toxin

• AlphaFold DB: U4SMA9