Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1650253..1650850 Replicon chromosome
Accession NZ_CP029137
Organism Klebsiella pneumoniae strain AR376

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag CSC13_RS09785 Protein ID WP_004142563.1
Coordinates 1650253..1650570 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag CSC13_RS09790 Protein ID WP_004142561.1
Coordinates 1650563..1650850 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC13_RS09765 1645953..1647380 - 1428 WP_009308097.1 MFS transporter -
CSC13_RS09770 1647489..1648355 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
CSC13_RS09780 1649025..1650068 + 1044 WP_020325241.1 DUF2157 domain-containing protein -
CSC13_RS09785 1650253..1650570 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CSC13_RS09790 1650563..1650850 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
CSC13_RS09795 1651115..1651768 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
CSC13_RS09800 1651888..1652256 - 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
CSC13_RS09805 1652256..1652838 - 583 Protein_1539 TetR/AcrR family transcriptional regulator -
CSC13_RS09810 1653228..1654133 + 906 WP_064483910.1 MBL fold metallo-hydrolase -
CSC13_RS09815 1654164..1654505 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
CSC13_RS09820 1654523..1654771 - 249 WP_020325243.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T105034 WP_004142563.1 NZ_CP029137:1650253-1650570 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T105034 NZ_CP029137:1650253-1650570 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT105034 WP_004142561.1 NZ_CP029137:1650563-1650850 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT105034 NZ_CP029137:1650563-1650850 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References