Detailed information of TA system TA104986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 70793..71450
• Replicon: plasmid unnamed1
• Accession: NZ_CP029123
• Organism: Escherichia coli strain AR434

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: CSC21_RS24655
• Protein ID: WP_000270043.1
• Coordinates: 70793..71143 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSC21_RS24660
• Protein ID: WP_000124640.1
• Coordinates: 71148..71450 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC21_RS24615	67267..67764	-	498	WP_000062185.1	hypothetical protein	-
CSC21_RS24620	67767..68255	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
CSC21_RS24625	68352..68687	+	336	WP_000683477.1	hypothetical protein	-
CSC21_RS24630	68702..69172	-	471	WP_001281821.1	hypothetical protein	-
CSC21_RS24635	69165..69536	-	372	WP_000516918.1	hypothetical protein	-
CSC21_RS24640	69547..69741	-	195	WP_000343597.1	hypothetical protein	-
CSC21_RS24650	70082..70630	-	549	WP_001061573.1	hypothetical protein	-
CSC21_RS24655	70793..71143	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSC21_RS24660	71148..71450	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
CSC21_RS24665	71477..71770	-	294	WP_001239998.1	hypothetical protein	-
CSC21_RS24670	71859..72131	-	273	WP_001043046.1	HU family DNA-binding protein	-
CSC21_RS24675	72189..72716	-	528	WP_004201083.1	thermonuclease family protein	-
CSC21_RS25300	72719..73024	-	306	WP_171820680.1	hypothetical protein	-
CSC21_RS24685	72936..73793	-	858	WP_001167036.1	hypothetical protein	-
CSC21_RS24690	73780..74010	-	231	WP_000972665.1	hypothetical protein	-
CSC21_RS24695	74010..74528	-	519	WP_000210757.1	nitrite reductase	-
CSC21_RS24700	74525..74971	-	447	WP_000919343.1	hypothetical protein	-
CSC21_RS24705	74971..75330	-	360	WP_000422769.1	hypothetical protein	-
CSC21_RS24710	75388..75816	-	429	WP_000591076.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aac(3)-IId / sul1 / qacE / blaVEB-5 / tet(A) / aac(6')-Ib	-	1..97471	97471

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T104986 WP_000270043.1 NZ_CP029123:70793-71143 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T104986 NZ_CP029123:70793-71143 [Escherichia coli]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT104986 WP_000124640.1 NZ_CP029123:71148-71450 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT104986 NZ_CP029123:71148-71450 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9