Detailed information of TA system TA104963

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26065..26329
• Replicon: plasmid unnamed6
• Accession: NZ_CP029119
• Organism: Escherichia coli strain AR435

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: CSC22_RS27945
• Protein ID: WP_001387489.1
• Coordinates: 26177..26329 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	26065..26125 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC22_RS27905	22190..22453	+	264	WP_032180564.1	ash family protein	-
CSC22_RS27910	22371..22658	+	288	WP_000074855.1	conjugal transfer protein TraA	-
CSC22_RS27920	23100..23405	+	306	Protein_31	transcription termination factor NusG	-
CSC22_RS27925	23419..24116	-	698	Protein_32	IS1 family transposase	-
CSC22_RS27935	24172..25329	-	1158	Protein_33	IS1380-like element ISEc9 family transposase	-
CSC22_RS27940	25515..25862	-	348	Protein_34	protein finQ	-
-	26065..26125	-	61	NuclAT_0	-	Antitoxin
-	26065..26125	-	61	NuclAT_0	-	Antitoxin
-	26065..26125	-	61	NuclAT_0	-	Antitoxin
-	26065..26125	-	61	NuclAT_0	-	Antitoxin
CSC22_RS27945	26177..26329	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
CSC22_RS27950	26401..26652	-	252	WP_001291964.1	hypothetical protein	-
CSC22_RS27955	26952..27248	+	297	WP_011264046.1	hypothetical protein	-
CSC22_RS28730	27313..27489	-	177	WP_001054897.1	hypothetical protein	-
CSC22_RS27960	27881..28090	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
CSC22_RS27965	28162..28824	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
CSC22_RS27970	28895..30649	-	1755	Protein_41	DotA/TraY family protein	-
CSC22_RS28485	30648..30764	-	117	Protein_42	IncI1-type conjugal transfer lipoprotein TraH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..35284	35284

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T104963 WP_001387489.1 NZ_CP029119:26177-26329 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T104963 NZ_CP029119:26177-26329 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT104963 NZ_CP029119:c26125-26065 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin