Detailed information of TA system TA104962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 36000..36657
• Replicon: plasmid unnamed5
• Accession: NZ_CP029118
• Organism: Escherichia coli strain AR435

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: CSC22_RS26795
• Protein ID: WP_000270043.1
• Coordinates: 36000..36350 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSC22_RS26800
• Protein ID: WP_000124640.1
• Coordinates: 36355..36657 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC22_RS26755	32474..32971	-	498	WP_000062185.1	hypothetical protein	-
CSC22_RS26760	32974..33462	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
CSC22_RS26765	33559..33894	+	336	WP_000683477.1	hypothetical protein	-
CSC22_RS26770	33909..34379	-	471	WP_001281821.1	hypothetical protein	-
CSC22_RS26775	34372..34743	-	372	WP_000516918.1	hypothetical protein	-
CSC22_RS26780	34754..34948	-	195	WP_000343597.1	hypothetical protein	-
CSC22_RS26790	35289..35837	-	549	WP_001061573.1	hypothetical protein	-
CSC22_RS26795	36000..36350	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSC22_RS26800	36355..36657	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
CSC22_RS26805	36684..36977	-	294	WP_001239998.1	hypothetical protein	-
CSC22_RS26810	37066..37338	-	273	WP_001043046.1	HU family DNA-binding protein	-
CSC22_RS26815	37396..37923	-	528	WP_001210659.1	thermonuclease family protein	-
CSC22_RS28715	37926..38231	-	306	WP_171820680.1	hypothetical protein	-
CSC22_RS26825	38143..39000	-	858	WP_001167036.1	hypothetical protein	-
CSC22_RS26830	38987..39217	-	231	WP_000972665.1	hypothetical protein	-
CSC22_RS26835	39217..39735	-	519	WP_000210757.1	nitrite reductase	-
CSC22_RS26840	39732..40178	-	447	WP_000919343.1	hypothetical protein	-
CSC22_RS26845	40178..40537	-	360	WP_000422769.1	hypothetical protein	-
CSC22_RS26850	40595..41023	-	429	WP_000591076.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaSHV-12 / blaNDM-1 / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12	htpB	1..187909	187909

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T104962 WP_000270043.1 NZ_CP029118:36000-36350 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T104962 NZ_CP029118:36000-36350 [Escherichia coli]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT104962 WP_000124640.1 NZ_CP029118:36355-36657 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT104962 NZ_CP029118:36355-36657 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9