Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2975799..2976301 Replicon chromosome
Accession NZ_CP029115
Organism Escherichia coli strain AR435

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CSC22_RS16260 Protein ID WP_000767829.1
Coordinates 2975799..2976053 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CSC22_RS16265 Protein ID WP_001259255.1
Coordinates 2976050..2976301 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC22_RS16230 2970814..2971041 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CSC22_RS16235 2971055..2972113 - 1059 WP_000492321.1 transport protein YeeE -
CSC22_RS16240 2972292..2973650 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CSC22_RS16245 2973640..2973702 - 63 WP_010723108.1 membrane protein YoeI -
CSC22_RS16250 2973917..2974846 - 930 WP_000803351.1 LysR family transcriptional regulator -
CSC22_RS16255 2974892..2975716 - 825 WP_000754749.1 SDR family oxidoreductase -
CSC22_RS16260 2975799..2976053 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CSC22_RS16265 2976050..2976301 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CSC22_RS16270 2976585..2976635 + 51 WP_001364200.1 his operon leader peptide -
CSC22_RS16280 2976781..2977680 + 900 WP_032329340.1 ATP phosphoribosyltransferase -
CSC22_RS16285 2977686..2978990 + 1305 WP_001446927.1 histidinol dehydrogenase -
CSC22_RS16290 2978987..2980057 + 1071 WP_000108945.1 histidinol-phosphate transaminase -
CSC22_RS16295 2980057..2981124 + 1068 WP_046464156.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T104954 WP_000767829.1 NZ_CP029115:c2976053-2975799 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T104954 NZ_CP029115:c2976053-2975799 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT104954 WP_001259255.1 NZ_CP029115:c2976301-2976050 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT104954 NZ_CP029115:c2976301-2976050 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References