Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1079994..1080688 Replicon chromosome
Accession NZ_CP029115
Organism Escherichia coli strain AR435

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag CSC22_RS06210 Protein ID WP_001263489.1
Coordinates 1080290..1080688 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag CSC22_RS06205 Protein ID WP_000554758.1
Coordinates 1079994..1080287 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC22_RS06185 1075626..1076123 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
CSC22_RS06190 1076347..1078059 - 1713 Protein_977 flagellar biosynthesis protein FlhA -
CSC22_RS06195 1078031..1078816 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
CSC22_RS06200 1078887..1079942 + 1056 WP_001226164.1 DNA polymerase IV -
CSC22_RS06205 1079994..1080287 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CSC22_RS06210 1080290..1080688 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CSC22_RS06215 1080698..1081150 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
CSC22_RS06220 1081468..1081674 + 207 Protein_983 RtcB family protein -
CSC22_RS06225 1081670..1082191 + 522 Protein_984 peptide chain release factor H -
CSC22_RS06230 1082248..1083705 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
CSC22_RS06235 1083966..1084424 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 1085020..1085100 + 81 NuclAT_11 - -
- 1085020..1085100 + 81 NuclAT_11 - -
- 1085020..1085100 + 81 NuclAT_11 - -
- 1085020..1085100 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T104940 WP_001263489.1 NZ_CP029115:1080290-1080688 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T104940 NZ_CP029115:1080290-1080688 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT104940 WP_000554758.1 NZ_CP029115:1079994-1080287 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT104940 NZ_CP029115:1079994-1080287 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References