Detailed information of TA system TA104929

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 14037..14458
• Replicon: plasmid unnamed1
• Accession: NZ_CP029113
• Organism: Escherichia coli strain AR435

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: CSC22_RS00100
• Protein ID: WP_001302184.1
• Coordinates: 14300..14458 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	14037..14235 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC22_RS00055	9733..11127	+	1395	WP_001037799.1	carbohydrate porin	-
CSC22_RS00065	11373..11477	-	105	Protein_8	thiosulfate reductase cytochrome B subunit	-
CSC22_RS00075	11534..12227	+	694	Protein_9	IS1 family transposase	-
CSC22_RS00080	12243..12866	+	624	WP_000912556.1	potassium channel family protein	-
CSC22_RS00085	12951..13202	+	252	Protein_11	iron-containing alcohol dehydrogenase	-
CSC22_RS00090	13491..13808	-	318	Protein_12	IS110 family transposase	-
CSC22_RS00095	13963..14068	+	106	Protein_13	plasmid SOS inhibition protein A	-
-	14037..14235	+	199	NuclAT_0	-	Antitoxin
-	14037..14235	+	199	NuclAT_0	-	Antitoxin
-	14037..14235	+	199	NuclAT_0	-	Antitoxin
-	14037..14235	+	199	NuclAT_0	-	Antitoxin
CSC22_RS00100	14300..14458	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
CSC22_RS28490	15126..15332	+	207	WP_000275854.1	hypothetical protein	-
CSC22_RS00110	15357..15644	+	288	WP_000107242.1	hypothetical protein	-
CSC22_RS00115	15763..16584	+	822	WP_001234465.1	DUF945 domain-containing protein	-
CSC22_RS00120	16880..17452	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
CSC22_RS00125	17822..18205	+	384	WP_001063020.1	relaxosome protein TraM	-
CSC22_RS00130	18396..19043	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
CSC22_RS00135	19179..19394	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aadA5 / qacE / sul1 / mph(A)	-	1..114412	114412

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T104929 WP_001302184.1 NZ_CP029113:14300-14458 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T104929 NZ_CP029113:14300-14458 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT104929 NZ_CP029113:14037-14235 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin