Detailed information of TA system TA104874

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 611..865
• Replicon: plasmid unnamed5
• Accession: NZ_CP029107
• Organism: Escherichia coli strain AR437

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CSC24_RS01145
• Protein ID: WP_015387399.1
• Coordinates: 659..865 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	611..672 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC24_RS01135	290..472	+	183	WP_000968309.1	hypothetical protein	-
-	611..672	-	62	NuclAT_0	-	Antitoxin
-	611..672	-	62	NuclAT_0	-	Antitoxin
-	611..672	-	62	NuclAT_0	-	Antitoxin
-	611..672	-	62	NuclAT_0	-	Antitoxin
CSC24_RS01145	659..865	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
CSC24_RS01150	1149..1406	+	258	WP_000083833.1	replication regulatory protein RepA	-
CSC24_RS01160	1642..1716	+	75	WP_032336874.1	RepA leader peptide Tap	-
CSC24_RS01165	1709..2155	+	447	Protein_5	incFII family plasmid replication initiator RepA	-
CSC24_RS01170	2101..2583	-	483	Protein_6	VENN motif pre-toxin domain-containing protein	-
CSC24_RS01175	3476..4696	+	1221	WP_000410951.1	arginine deiminase	-
CSC24_RS01180	4707..5618	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / mph(A) / sitABCD	iucA / iucB / iucC / iucD / iutA	1..93435	93435

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T104874 WP_015387399.1 NZ_CP029107:659-865 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T104874 NZ_CP029107:659-865 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT104874 NZ_CP029107:c672-611 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin