Detailed information of TA system TA104846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1290977..1291572
• Replicon: chromosome
• Accession: NZ_CP029097
• Organism: Pseudomonas aeruginosa strain AR439

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: CSC26_RS08500
• Protein ID: WP_003117425.1
• Coordinates: 1290977..1291255 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSC26_RS08505
• Protein ID: WP_003099268.1
• Coordinates: 1291267..1291572 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC26_RS08480	1286403..1287641	+	1239	WP_014603324.1	C69 family dipeptidase	-
CSC26_RS08485	1287703..1288350	+	648	WP_003095021.1	carbonate dehydratase	-
CSC26_RS08490	1288420..1290648	-	2229	WP_003099265.1	TonB-dependent receptor	-
CSC26_RS08500	1290977..1291255	+	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSC26_RS08505	1291267..1291572	+	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
CSC26_RS08510	1291949..1292476	+	528	WP_071535723.1	AAA family ATPase	-
CSC26_RS08515	1292488..1294026	-	1539	WP_023082696.1	GNAT family N-acetyltransferase	-
CSC26_RS08520	1294001..1294846	-	846	WP_196645791.1	helix-turn-helix domain-containing protein	-
CSC26_RS08525	1294969..1295235	-	267	WP_016852153.1	hypothetical protein	-
CSC26_RS08530	1295345..1295617	+	273	WP_003115921.1	hypothetical protein	-
CSC26_RS08535	1295610..1295825	+	216	WP_031629304.1	hypothetical protein	-
CSC26_RS08540	1295829..1296119	+	291	WP_023083237.1	DUF5447 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T104846 WP_003117425.1 NZ_CP029097:1290977-1291255 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T104846 NZ_CP029097:1290977-1291255 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT104846 WP_003099268.1 NZ_CP029097:1291267-1291572 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT104846 NZ_CP029097:1291267-1291572 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5