Detailed information of TA system TA104839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5140394..5140989
• Replicon: chromosome
• Accession: NZ_CP029090
• Organism: Pseudomonas aeruginosa strain AR442

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: CSC29_RS25280
• Protein ID: WP_003117425.1
• Coordinates: 5140711..5140989 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSC29_RS25275
• Protein ID: WP_003099268.1
• Coordinates: 5140394..5140699 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC29_RS25240	5135532..5136380	+	849	WP_003117426.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
CSC29_RS25250	5136547..5137488	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
CSC29_RS25255	5137605..5138219	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
CSC29_RS25260	5138262..5138846	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
CSC29_RS25265	5138887..5139987	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
CSC29_RS25275	5140394..5140699	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
CSC29_RS25280	5140711..5140989	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSC29_RS25290	5141318..5143546	+	2229	WP_003099265.1	TonB-dependent receptor	-
CSC29_RS25295	5143616..5144263	-	648	WP_003095021.1	carbonate dehydratase	-
CSC29_RS25300	5144325..5145563	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T104839 WP_003117425.1 NZ_CP029090:c5140989-5140711 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T104839 NZ_CP029090:c5140989-5140711 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT104839 WP_003099268.1 NZ_CP029090:c5140699-5140394 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT104839 NZ_CP029090:c5140699-5140394 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5