Detailed information of TA system TA104825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6044391..6044986
• Replicon: chromosome
• Accession: NZ_CP029088
• Organism: Pseudomonas aeruginosa strain AR445

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: CSC32_RS29415
• Protein ID: WP_003117425.1
• Coordinates: 6044708..6044986 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSC32_RS29410
• Protein ID: WP_003099268.1
• Coordinates: 6044391..6044696 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC32_RS29365	6039614..6039865	-	252	WP_003115979.1	hypothetical protein	-
CSC32_RS29375	6039987..6040421	-	435	WP_023087663.1	hypothetical protein	-
CSC32_RS29385	6040937..6041227	-	291	WP_033896069.1	DUF5447 family protein	-
CSC32_RS29390	6041403..6041675	-	273	WP_004352675.1	hypothetical protein	-
CSC32_RS29400	6042122..6042457	+	336	WP_025297674.1	hypothetical protein	-
CSC32_RS34635	6042510..6043601	+	1092	WP_124135598.1	hypothetical protein	-
CSC32_RS29405	6043662..6044054	-	393	WP_025297673.1	hypothetical protein	-
CSC32_RS29410	6044391..6044696	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
CSC32_RS29415	6044708..6044986	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSC32_RS29425	6045311..6047539	+	2229	WP_003113525.1	TonB-dependent receptor	-
CSC32_RS29430	6047609..6048256	-	648	WP_003095021.1	carbonate dehydratase	-
CSC32_RS29435	6048318..6049556	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T104825 WP_003117425.1 NZ_CP029088:c6044986-6044708 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T104825 NZ_CP029088:c6044986-6044708 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT104825 WP_003099268.1 NZ_CP029088:c6044696-6044391 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT104825 NZ_CP029088:c6044696-6044391 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5