Detailed information of TA system TA104795

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 996215..996732
• Replicon: chromosome
• Accession: NZ_CP029086
• Organism: Staphylococcus aureus strain AR462

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: CSC48_RS05500
• Protein ID: WP_000113265.1
• Coordinates: 996215..996481 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: CSC48_RS05505
• Protein ID: WP_000584499.1
• Coordinates: 996481..996732 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC48_RS05470	991643..992179	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
CSC48_RS05475	992413..993237	-	825	WP_000572038.1	formate/nitrite transporter family protein	-
CSC48_RS05480	993454..993636	-	183	WP_000230292.1	hypothetical protein	-
CSC48_RS05485	993720..994187	-	468	WP_000153347.1	SRPBCC domain-containing protein	-
CSC48_RS05490	994373..995920	-	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
CSC48_RS05495	996109..996180	-	72	Protein_967	hypothetical protein	-
CSC48_RS05500	996215..996481	-	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
CSC48_RS05505	996481..996732	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CSC48_RS05510	996897..996998	-	102	Protein_970	hypothetical protein	-
CSC48_RS05515	997005..997604	-	600	WP_000162810.1	DsbA family protein	-
CSC48_RS05520	997623..997985	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
CSC48_RS05525	998240..999490	+	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
CSC48_RS05530	999587..1000318	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
CSC48_RS05535	1000315..1001034	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T104795 WP_000113265.1 NZ_CP029086:c996481-996215 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T104795 NZ_CP029086:c996481-996215 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT104795 WP_000584499.1 NZ_CP029086:c996732-996481 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT104795 NZ_CP029086:c996732-996481 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60