Detailed information of TA system TA104760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1228927..1229444
• Replicon: chromosome
• Accession: NZ_CP029082
• Organism: Staphylococcus aureus strain AR465

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: CSC50_RS06355
• Protein ID: WP_000113265.1
• Coordinates: 1229178..1229444 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: CSC50_RS06350
• Protein ID: WP_000584499.1
• Coordinates: 1228927..1229178 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC50_RS06320	1224625..1225344	+	720	WP_000479576.1	amino acid ABC transporter permease	-
CSC50_RS06325	1225341..1226072	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
CSC50_RS06330	1226169..1227419	-	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
CSC50_RS06335	1227674..1228036	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
CSC50_RS06340	1228055..1228654	+	600	WP_000162810.1	DsbA family protein	-
CSC50_RS06345	1228661..1228762	+	102	Protein_1149	hypothetical protein	-
CSC50_RS06350	1228927..1229178	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CSC50_RS06355	1229178..1229444	+	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
CSC50_RS06360	1229479..1229550	+	72	Protein_1152	hypothetical protein	-
CSC50_RS06365	1229739..1231286	+	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
CSC50_RS06370	1231472..1231939	+	468	WP_000153347.1	SRPBCC domain-containing protein	-
CSC50_RS06375	1232023..1232205	+	183	WP_000230292.1	hypothetical protein	-
CSC50_RS06380	1232422..1233246	+	825	WP_000572038.1	formate/nitrite transporter family protein	-
CSC50_RS06385	1233480..1234016	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T104760 WP_000113265.1 NZ_CP029082:1229178-1229444 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T104760 NZ_CP029082:1229178-1229444 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT104760 WP_000584499.1 NZ_CP029082:1228927-1229178 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT104760 NZ_CP029082:1228927-1229178 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60