Detailed information of TA system TA104733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3518754..3519424
• Replicon: chromosome
• Accession: NZ_CP029065
• Organism: Mycobacterium tuberculosis strain TBMENG-03

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: DDF83_RS17340
• Protein ID: WP_003899954.1
• Coordinates: 3518754..3519098 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: DDF83_RS17345
• Protein ID: WP_003899955.1
• Coordinates: 3519095..3519424 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDF83_RS17305	3513827..3514687	+	861	WP_003416628.1	alpha/beta hydrolase	-
DDF83_RS17310	3514662..3515177	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
DDF83_RS17320	3515417..3515710	+	294	WP_003416635.1	hypothetical protein	-
DDF83_RS17325	3515998..3517287	+	1290	WP_003416640.1	ATP-binding protein	-
DDF83_RS17330	3517634..3518068	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
DDF83_RS17335	3518071..3518523	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
DDF83_RS17340	3518754..3519098	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DDF83_RS17345	3519095..3519424	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
DDF83_RS17350	3520073..3520420	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
DDF83_RS17355	3520417..3521037	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
DDF83_RS17360	3521197..3522462	-	1266	Protein_3277	hypothetical protein	-
DDF83_RS17365	3522630..3522839	+	210	WP_003416778.1	hypothetical protein	-
DDF83_RS17375	3523086..3524120	-	1035	WP_003905011.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T104733 WP_003899954.1 NZ_CP029065:3518754-3519098 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T104733 NZ_CP029065:3518754-3519098 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT104733 WP_003899955.1 NZ_CP029065:3519095-3519424 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT104733 NZ_CP029065:3519095-3519424 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6