Detailed information of TA system TA104668

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 160920..161184
• Replicon: plasmid pFORC_081_1
• Accession: NZ_CP029058
• Organism: Escherichia coli strain FORC_081

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: FORC81_RS24315
• Protein ID: WP_001331364.1
• Coordinates: 160920..161072 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	161127..161184 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC81_RS24295	156197..158365	+	2169	WP_021537350.1	DotA/TraY family protein	-
FORC81_RS24300	158439..159089	+	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
FORC81_RS24305	159161..159370	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
FORC81_RS25825	159762..159938	+	177	WP_001054904.1	hypothetical protein	-
FORC81_RS24310	160597..160848	+	252	WP_001291964.1	hypothetical protein	-
FORC81_RS24315	160920..161072	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	161127..161184	+	58	NuclAT_1	-	Antitoxin
-	161127..161184	+	58	NuclAT_1	-	Antitoxin
-	161127..161184	+	58	NuclAT_1	-	Antitoxin
-	161127..161184	+	58	NuclAT_1	-	Antitoxin
FORC81_RS24320	161364..162572	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
FORC81_RS24325	162591..163661	+	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
FORC81_RS24330	163654..165945	+	2292	WP_001289276.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / qnrS1 / tet(A) / blaTEM-1B / aac(3)-IId / floR	-	1..253947	253947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T104668 WP_001331364.1 NZ_CP029058:c161072-160920 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T104668 NZ_CP029058:c161072-160920 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT104668 NZ_CP029058:161127-161184 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin