Detailed information of TA system TA104664

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 64911..65337
• Replicon: plasmid pFORC_081_1
• Accession: NZ_CP029058
• Organism: Escherichia coli strain FORC_081

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FORC81_RS23670
• Protein ID: WP_001312861.1
• Coordinates: 64911..65069 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	65113..65337 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC81_RS23620	59962..60189	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
FORC81_RS23625	60283..60969	-	687	WP_000332484.1	PAS domain-containing protein	-
FORC81_RS23630	61160..61543	-	384	WP_000124981.1	relaxosome protein TraM	-
FORC81_RS23635	61820..62467	+	648	WP_072101530.1	transglycosylase SLT domain-containing protein	-
FORC81_RS23645	62762..63583	-	822	WP_001234469.1	DUF945 domain-containing protein	-
FORC81_RS23650	63702..63989	-	288	WP_000107542.1	hypothetical protein	-
FORC81_RS25955	64014..64220	-	207	WP_000275853.1	hypothetical protein	-
FORC81_RS23670	64911..65069	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	65113..65337	-	225	NuclAT_0	-	Antitoxin
-	65113..65337	-	225	NuclAT_0	-	Antitoxin
-	65113..65337	-	225	NuclAT_0	-	Antitoxin
-	65113..65337	-	225	NuclAT_0	-	Antitoxin
FORC81_RS25815	65149..65337	+	189	WP_001299721.1	hypothetical protein	-
FORC81_RS23675	65349..66068	-	720	WP_001276235.1	plasmid SOS inhibition protein A	-
FORC81_RS23680	66065..66499	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FORC81_RS23685	66554..68512	-	1959	WP_004023025.1	ParB/RepB/Spo0J family partition protein	-
FORC81_RS23690	68571..68804	-	234	WP_000006004.1	DUF905 domain-containing protein	-
FORC81_RS23695	68860..69387	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
FORC81_RS23715	69860..70120	-	261	WP_112001249.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / qnrS1 / tet(A) / blaTEM-1B / aac(3)-IId / floR	-	1..253947	253947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T104664 WP_001312861.1 NZ_CP029058:c65069-64911 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T104664 NZ_CP029058:c65069-64911 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT104664 NZ_CP029058:c65337-65113 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin