Detailed information of TA system TA104655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 2444741..2445282
• Replicon: chromosome
• Accession: NZ_CP029057
• Organism: Escherichia coli strain FORC_081

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: Q47149
• Locus tag: FORC81_RS12185
• Protein ID: WP_000615983.1
• Coordinates: 2445004..2445282 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: Q47150
• Locus tag: FORC81_RS12180
• Protein ID: WP_000729703.1
• Coordinates: 2444741..2445001 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC81_RS12165	2440792..2442885	+	2094	WP_001140826.1	flagellar biosynthesis protein FlhA	-
FORC81_RS12170	2443109..2443606	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
FORC81_RS12175	2443782..2444531	-	750	WP_000056849.1	C40 family peptidase	-
FORC81_RS12180	2444741..2445001	+	261	WP_000729703.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
FORC81_RS12185	2445004..2445282	+	279	WP_000615983.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
FORC81_RS12190	2445438..2446178	+	741	WP_001225679.1	murein L,D-transpeptidase	-
FORC81_RS12195	2446149..2446916	-	768	WP_000333380.1	class II glutamine amidotransferase	-
FORC81_RS12200	2447122..2447700	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / rhs/PAAR / tssA / hcp1/tssD1	2443782..2467528	23746

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10846.62 Da
• Isoelectric Point: 9.9941

>T104655 WP_000615983.1 NZ_CP029057:2445004-2445282 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T104655 NZ_CP029057:2445004-2445282 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9405.80 Da
• Isoelectric Point: 5.0758

>AT104655 WP_000729703.1 NZ_CP029057:2444741-2445001 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT104655 NZ_CP029057:2444741-2445001 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• PDB: 4Q2U

Antitoxin

• AlphaFold DB: A0A5F1F716