Detailed information of TA system TA104654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2403080..2403774
• Replicon: chromosome
• Accession: NZ_CP029057
• Organism: Escherichia coli strain FORC_081

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: FORC81_RS11945
• Protein ID: WP_001263489.1
• Coordinates: 2403080..2403478 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: FORC81_RS11950
• Protein ID: WP_000554758.1
• Coordinates: 2403481..2403774 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	2398668..2398748	-	81	NuclAT_12	-	-
-	2398668..2398748	-	81	NuclAT_12	-	-
-	2398668..2398748	-	81	NuclAT_12	-	-
-	2398668..2398748	-	81	NuclAT_12	-	-
FORC81_RS11920	2399344..2399802	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FORC81_RS11925	2400063..2401520	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
FORC81_RS11930	2401577..2402098	-	522	Protein_2302	peptide chain release factor H	-
FORC81_RS11935	2402094..2402300	-	207	Protein_2303	RtcB family protein	-
FORC81_RS11940	2402618..2403070	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
FORC81_RS11945	2403080..2403478	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FORC81_RS11950	2403481..2403774	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FORC81_RS11955	2403826..2404881	-	1056	WP_001226164.1	DNA polymerase IV	-
FORC81_RS11960	2404952..2405875	-	924	WP_001232546.1	putative lateral flagellar export/assembly protein LafU	-
FORC81_RS11965	2405878..2406741	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
FORC81_RS11970	2406754..2407470	-	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
FORC81_RS11975	2407490..2407957	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T104654 WP_001263489.1 NZ_CP029057:c2403478-2403080 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T104654 NZ_CP029057:c2403478-2403080 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT104654 WP_000554758.1 NZ_CP029057:c2403774-2403481 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT104654 NZ_CP029057:c2403774-2403481 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3