Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 9822..10473 | Replicon | plasmid pAbW39-2 |
| Accession | NZ_CP029007 | ||
| Organism | Acinetobacter pittii strain AbW39 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | S3SZA7 |
| Locus tag | DDE02_RS20770 | Protein ID | WP_000269903.1 |
| Coordinates | 10117..10473 (-) | Length | 119 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | DDE02_RS20765 | Protein ID | WP_001140619.1 |
| Coordinates | 9822..10124 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DDE02_RS20740 (DDE02_21015) | 6040..6969 | + | 930 | WP_005065049.1 | replication initiation protein RepM | - |
| DDE02_RS20745 (DDE02_21020) | 7048..7578 | + | 531 | WP_006581721.1 | plasmid replication DNA-binding protein | - |
| DDE02_RS20750 (DDE02_21025) | 7669..8478 | + | 810 | Protein_11 | IS5 family transposase | - |
| DDE02_RS20755 (DDE02_21035) | 9283..9510 | + | 228 | WP_005071544.1 | hypothetical protein | - |
| DDE02_RS20760 (DDE02_21040) | 9620..9829 | - | 210 | WP_000069471.1 | hypothetical protein | - |
| DDE02_RS20765 (DDE02_21045) | 9822..10124 | - | 303 | WP_001140619.1 | XRE family transcriptional regulator | Antitoxin |
| DDE02_RS20770 (DDE02_21050) | 10117..10473 | - | 357 | WP_000269903.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| DDE02_RS20775 (DDE02_21055) | 11256..11585 | + | 330 | WP_000610966.1 | four-helix bundle copper-binding protein | - |
| DDE02_RS20780 (DDE02_21060) | 11837..12199 | - | 363 | Protein_17 | plasmid replication DNA-binding protein | - |
| DDE02_RS20785 | 12233..12457 | - | 225 | WP_126026108.1 | hypothetical protein | - |
| DDE02_RS20790 | 12607..12702 | - | 96 | Protein_19 | DNA-binding protein | - |
| DDE02_RS20795 (DDE02_21065) | 12700..12870 | - | 171 | Protein_20 | IS3 family transposase | - |
| DDE02_RS20800 (DDE02_21070) | 13046..13330 | - | 285 | WP_005071539.1 | putative quinol monooxygenase | - |
| DDE02_RS20805 (DDE02_21075) | 13350..14348 | - | 999 | WP_005071537.1 | zinc-binding alcohol dehydrogenase family protein | - |
| DDE02_RS20810 (DDE02_21080) | 14421..15161 | - | 741 | WP_005071535.1 | SDR family oxidoreductase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..149504 | 149504 | |
| - | flank | IS/Tn | - | - | 7726..8478 | 752 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 119 a.a. Molecular weight: 13486.45 Da Isoelectric Point: 7.1645
>T104588 WP_000269903.1 NZ_CP029007:c10473-10117 [Acinetobacter pittii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG
Download Length: 357 bp
>T104588 NZ_CP029007:c10473-10117 [Acinetobacter pittii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAAAT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAAAT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11206.01 Da Isoelectric Point: 6.7282
>AT104588 WP_001140619.1 NZ_CP029007:c10124-9822 [Acinetobacter pittii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV
Download Length: 303 bp
>AT104588 NZ_CP029007:c10124-9822 [Acinetobacter pittii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAATTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTCTAA
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAATTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTCTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|