Detailed information of TA system TA104586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 213829..214414
• Replicon: plasmid pAbW39-1
• Accession: NZ_CP029006
• Organism: Acinetobacter pittii strain AbW39

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7S7BY50
• Locus tag: DDE02_RS20410
• Protein ID: WP_000897311.1
• Coordinates: 213829..214149 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DDE02_RS20415
• Protein ID: WP_000369784.1
• Coordinates: 214142..214414 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDE02_RS20390 (DDE02_20625)	209636..209965	+	330	WP_000506885.1	DUF4236 domain-containing protein	-
DDE02_RS20395 (DDE02_20635)	210237..211469	-	1233	WP_001255535.1	ATP-binding protein	-
DDE02_RS20400 (DDE02_20645)	211727..212164	-	438	Protein_211	IS3 family transposase	-
DDE02_RS20405 (DDE02_20650)	212218..213150	+	933	WP_252618458.1	IS5-like element IS17 family transposase	-
DDE02_RS20410 (DDE02_20655)	213829..214149	+	321	WP_000897311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DDE02_RS20415 (DDE02_20660)	214142..214414	+	273	WP_000369784.1	NadS family protein	Antitoxin
DDE02_RS20420 (DDE02_20665)	214447..215247	+	801	WP_000604996.1	DUF2971 domain-containing protein	-
DDE02_RS20425 (DDE02_20670)	215338..215639	+	302	Protein_216	IS3 family transposase	-
DDE02_RS20430 (DDE02_20675)	215755..215967	+	213	WP_001123161.1	hypothetical protein	-
DDE02_RS20435 (DDE02_20680)	216293..217150	-	858	WP_000609033.1	LysR family transcriptional regulator	-
DDE02_RS20440 (DDE02_20685)	217147..218154	-	1008	WP_005071472.1	phosphonate dehydrogenase	-
DDE02_RS20445 (DDE02_20690)	218196..218993	-	798	WP_004757464.1	phosphonate ABC transporter, permease protein PhnE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(6)-Id / aph(3'')-Ib	-	1..262817	262817
	-	inside	IScluster/Tn	-	-	211727..215673	3946

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-84)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12214.03 Da
• Isoelectric Point: 7.9500

>T104586 WP_000897311.1 NZ_CP029006:213829-214149 [Acinetobacter pittii]
MLFIETSIFTKQIKELVTDEEYRQLQQDLLIQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFNG

Nucleotide

• Length: 321 bp

>T104586 NZ_CP029006:213829-214149 [Acinetobacter pittii]
ATGTTATTCATTGAAACCAGTATTTTTACCAAGCAAATCAAAGAGCTTGTTACTGATGAAGAATATCGTCAACTCCAGCA
AGATCTATTGATACAACCTGATAAAGGAGACTTGATCAAAAATGGTGGTGGCATTCGTAAAGTCCGTTGTGCTCAGGGTA
ATAAAGGCAAAAGTGGCGGTATTCGTGTGATCTATTACTGGGTTACAGAGGATGATCAGATTTTTTTCCTAGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAGACCTCGATCCTGCGTCAATTAGTGAAGGAGCAATTTAATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10145.57 Da
• Isoelectric Point: 7.0146

>AT104586 WP_000369784.1 NZ_CP029006:214142-214414 [Acinetobacter pittii]
MDNNLFDDLVTSIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDTNPTLI

Nucleotide

• Length: 273 bp

>AT104586 NZ_CP029006:214142-214414 [Acinetobacter pittii]
ATGGATAACAACTTATTTGATGACCTCGTTACTTCCATCAAAGAAGCTGGAGCCATCAAACGTAATGAGATAAAGGCAAG
TCGAGTAACAGAACTTGAATTGCCTGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCAGAGTTTGCTGCAC
GCTTACATATTAGCGCGAGAACTCTACAAAATTGGGAACAAGGTCGTCGCTATCCGACTGGCCCAGCAGCGACTTTGATT
CGTATTCTTGATACCAATCCAACTCTGATTTAG

Structures

Toxin

• AlphaFold DB: A0A7S7BY50