Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 162177..162834 | Replicon | plasmid unnamed5 |
| Accession | NZ_CP028996 | ||
| Organism | Klebsiella pneumoniae strain AR_0079 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | AM458_RS30235 | Protein ID | WP_000270043.1 |
| Coordinates | 162484..162834 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | AM458_RS30230 | Protein ID | WP_000124640.1 |
| Coordinates | 162177..162479 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AM458_RS30180 | 157802..158230 | + | 429 | WP_000591074.1 | hypothetical protein | - |
| AM458_RS30185 | 158287..158646 | + | 360 | WP_000422768.1 | hypothetical protein | - |
| AM458_RS30190 | 158646..159092 | + | 447 | WP_000919345.1 | Fe3+-siderophore ABC transporter permease | - |
| AM458_RS30195 | 159089..159607 | + | 519 | WP_000210756.1 | nitrite reductase | - |
| AM458_RS30200 | 159607..159837 | + | 231 | WP_000972663.1 | hypothetical protein | - |
| AM458_RS30205 | 159824..160681 | + | 858 | WP_001167032.1 | hypothetical protein | - |
| AM458_RS30215 | 160912..161439 | + | 528 | WP_001236377.1 | thermonuclease family protein | - |
| AM458_RS30220 | 161497..161769 | + | 273 | WP_001043047.1 | HU family DNA-binding protein | - |
| AM458_RS30225 | 161857..162150 | + | 294 | WP_001239997.1 | chromosome segregation protein ParM | - |
| AM458_RS30230 | 162177..162479 | - | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| AM458_RS30235 | 162484..162834 | - | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| AM458_RS30240 | 162997..163545 | + | 549 | WP_001061574.1 | transcriptional regulator | - |
| AM458_RS30250 | 163886..164080 | + | 195 | WP_000343597.1 | hypothetical protein | - |
| AM458_RS30255 | 164091..164462 | + | 372 | WP_000516916.1 | hypothetical protein | - |
| AM458_RS30260 | 164455..164925 | + | 471 | WP_001281821.1 | hypothetical protein | - |
| AM458_RS30265 | 164940..165275 | - | 336 | WP_000683476.1 | hypothetical protein | - |
| AM458_RS30270 | 165372..165860 | + | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| AM458_RS30275 | 165863..166360 | + | 498 | WP_000062185.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | erm(42) / blaTEM-1B / rmtB / tet(G) / floR / qacE / aadA2 / dfrA12 / aac(3)-IId / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-14 | - | 1..176325 | 176325 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T104576 WP_000270043.1 NZ_CP028996:c162834-162484 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T104576 NZ_CP028996:c162834-162484 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT104576 WP_000124640.1 NZ_CP028996:c162479-162177 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT104576 NZ_CP028996:c162479-162177 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|