Detailed information of TA system TA104519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1493322..1493927
• Replicon: chromosome
• Accession: NZ_CP028962
• Organism: Burkholderia sp. IDO3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DCN14_RS06875
• Protein ID: WP_096498818.1
• Coordinates: 1493649..1493927 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2A4CLK3
• Locus tag: DCN14_RS06870
• Protein ID: WP_057930197.1
• Coordinates: 1493322..1493636 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DCN14_RS06860	1490578..1491342	-	765	WP_096498822.1	Crp/Fnr family transcriptional regulator	-
DCN14_RS06865	1491559..1493211	+	1653	WP_096498820.1	acyl-CoA synthetase	-
DCN14_RS06870	1493322..1493636	-	315	WP_057930197.1	HigA family addiction module antidote protein	Antitoxin
DCN14_RS06875	1493649..1493927	-	279	WP_096498818.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DCN14_RS06880	1494021..1494452	-	432	WP_096499311.1	DedA family protein	-
DCN14_RS06885	1494561..1495205	-	645	WP_096498816.1	LysE family translocator	-
DCN14_RS06890	1495492..1495974	+	483	WP_096498814.1	Lrp/AsnC family transcriptional regulator	-
DCN14_RS06900	1496793..1498445	-	1653	WP_096498810.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-98)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10484.85 Da
• Isoelectric Point: 7.7175

>T104519 WP_096498818.1 NZ_CP028962:c1493927-1493649 [Burkholderia sp. IDO3]
MIQSFKCKDTRQLFETGRCRQFANIADVAMRRLTQLDNAATLDAMKSPPGNRFEALKGDRVGQYSIRINGQWRVCFTWGD
DGPADVEIADYH

Nucleotide

• Length: 279 bp

>T104519 NZ_CP028962:c1493927-1493649 [Burkholderia sp. IDO3]
ATGATCCAGTCGTTCAAATGCAAAGACACGCGGCAGCTTTTCGAAACGGGACGTTGCAGACAATTCGCCAACATTGCCGA
TGTCGCAATGCGTCGTCTTACGCAACTTGACAACGCGGCGACGCTGGACGCGATGAAATCACCGCCCGGCAACCGATTTG
AGGCACTGAAGGGCGATCGTGTTGGCCAGTACAGCATCCGAATCAACGGCCAATGGCGTGTGTGCTTTACATGGGGCGAT
GACGGGCCGGCCGATGTGGAAATCGCCGACTATCACTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11785.46 Da
• Isoelectric Point: 9.6536

>AT104519 WP_057930197.1 NZ_CP028962:c1493636-1493322 [Burkholderia sp. IDO3]
MTRFKNGMRPVHPGEILREDYLIPLDMSANALAGALGVTAGRINDIVRERRGITADTALRLARYFGGDPQTWLNLQATYD
LKIAERDHGREIQKRIEPREFAHA

Nucleotide

• Length: 315 bp

>AT104519 NZ_CP028962:c1493636-1493322 [Burkholderia sp. IDO3]
ATGACCCGGTTCAAAAACGGTATGCGCCCGGTCCATCCGGGCGAAATTCTTCGTGAGGATTACCTCATCCCGCTCGACAT
GAGCGCGAACGCGTTGGCCGGCGCACTTGGCGTCACGGCCGGCCGCATCAACGATATCGTCCGTGAGCGGCGCGGCATCA
CCGCCGATACGGCGCTGCGCCTTGCCCGCTACTTTGGCGGCGATCCGCAAACGTGGCTGAACCTGCAAGCCACCTATGAC
CTGAAAATCGCCGAGCGCGACCACGGCCGCGAGATCCAGAAGCGCATCGAGCCCCGAGAATTCGCCCACGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2A4CLK3