Detailed information of TA system TA104501

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4364183..4364829
• Replicon: chromosome
• Accession: NZ_CP028953
• Organism: Klebsiella pneumoniae strain AR_0141

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM388_RS21935
• Protein ID: WP_023317328.1
• Coordinates: 4364183..4364530 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM388_RS21940
• Protein ID: WP_032412314.1
• Coordinates: 4364530..4364829 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM388_RS21925	4360109..4361542	+	1434	WP_004145113.1	glycogen synthase GlgA	-
AM388_RS21930	4361560..4364007	+	2448	WP_002920561.1	glycogen phosphorylase	-
AM388_RS21935	4364183..4364530	+	348	WP_023317328.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM388_RS21940	4364530..4364829	+	300	WP_032412314.1	XRE family transcriptional regulator	Antitoxin
AM388_RS21945	4364892..4366400	-	1509	WP_032412313.1	glycerol-3-phosphate dehydrogenase	-
AM388_RS21950	4366605..4366934	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
AM388_RS21955	4366985..4367815	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
AM388_RS21960	4367865..4368623	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13562.60 Da
• Isoelectric Point: 6.2327

>T104501 WP_023317328.1 NZ_CP028953:4364183-4364530 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
ILCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T104501 NZ_CP028953:4364183-4364530 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
ATTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10828.64 Da
• Isoelectric Point: 10.1464

>AT104501 WP_032412314.1 NZ_CP028953:4364530-4364829 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGIKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT104501 NZ_CP028953:4364530-4364829 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGATAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA