Detailed information of TA system TA104494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2583939..2584536
• Replicon: chromosome
• Accession: NZ_CP028953
• Organism: Klebsiella pneumoniae strain AR_0141

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: AM388_RS13055
• Protein ID: WP_004893639.1
• Coordinates: 2584219..2584536 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: AM388_RS13050
• Protein ID: WP_004142561.1
• Coordinates: 2583939..2584226 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM388_RS13015	2579655..2579798	-	144	Protein_2484	AraC family transcriptional regulator	-
AM388_RS13020	2579829..2580944	-	1116	Protein_2485	MBL fold metallo-hydrolase	-
AM388_RS13025	2581124..2581312	+	189	Protein_2486	helix-turn-helix transcriptional regulator	-
AM388_RS13030	2581376..2582080	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
AM388_RS13035	2582135..2582533	+	399	Protein_2488	TetR/AcrR family transcriptional regulator	-
AM388_RS13040	2582533..2582901	+	369	WP_032421096.1	MmcQ/YjbR family DNA-binding protein	-
AM388_RS13045	2583021..2583674	+	654	WP_068815271.1	oxygen-insensitive NAD(P)H nitroreductase	-
AM388_RS13050	2583939..2584226	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
AM388_RS13055	2584219..2584536	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM388_RS13060	2584721..2585764	-	1044	WP_032410657.1	DUF2157 domain-containing protein	-
AM388_RS13070	2586430..2587296	-	867	WP_068815272.1	helix-turn-helix transcriptional regulator	-
AM388_RS13075	2587405..2588832	+	1428	WP_009308097.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T104494 WP_004893639.1 NZ_CP028953:c2584536-2584219 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T104494 NZ_CP028953:c2584536-2584219 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT104494 WP_004142561.1 NZ_CP028953:c2584226-2583939 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT104494 NZ_CP028953:c2584226-2583939 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1